Cell growth on 3D microstructured surfaces.

Autor: Araujo WW; Institute of Physics, University of São Paulo, C.P. 66318, CEP 05315-970 São Paulo, SP, Brazil. Electronic address: wwlysses@if.usp.com., Teixeira FS; Institute of Physics, University of São Paulo, C.P. 66318, CEP 05315-970 São Paulo, SP, Brazil., da Silva GN; Pharmacy School, UFOP - Federal University of Ouro Preto, Ouro Preto, MG, Brazil., Salvadori DM; Botucatu Medical School, UNESP - São Paulo State University, Botucatu, SP, Brazil. Electronic address: mcsalva@if.usp.br., Salvadori MC; Institute of Physics, University of São Paulo, C.P. 66318, CEP 05315-970 São Paulo, SP, Brazil., Brown IG; Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, United States.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2016 Jun; Vol. 63, pp. 686-9. Date of Electronic Publication: 2016 Mar 14.
DOI: 10.1016/j.msec.2016.03.026
Abstrakt: Chinese Hamster Ovary (CHO) cell cultures were grown on surfaces lithographed with periodic 3D hexagonal microcavity array morphology. The range of microcavity size (inscribed circle diameter) was from 12 μm to 560 μm. CHO cells were grown also on flat surfaces. The characterization was performed with respect to cell growth density (number of nuclei per unit area) by fluorescence optical microscopy and evaluated by correlation function analysis. We found that optimum microcavity radius was 80 μm, concerning to the maximum cell growth density, being even greater than the growth density on a flat (unstructured) substrate of the same material. This finding can be important for optimization of biotechnological processes and devices.
(Copyright © 2016. Published by Elsevier B.V.)
Databáze: MEDLINE
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