DNA Origami Seesaws as Comparative Binding Assay.

Autor: Nickels PC; Faculty of Physics and Center for NanoScience, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539, Munich, Germany., Høiberg HC; Faculty of Physics and Center for NanoScience, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539, Munich, Germany., Simmel SS; Faculty of Physics and Center for NanoScience, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539, Munich, Germany., Holzmeister P; Institut für physikalische und theoretische Chemie, Technische Universität Braunschweig, Hans-Sommer-Strasse 10, 38106, Braunschweig, Germany., Tinnefeld P; Institut für physikalische und theoretische Chemie, Technische Universität Braunschweig, Hans-Sommer-Strasse 10, 38106, Braunschweig, Germany., Liedl T; Faculty of Physics and Center for NanoScience, Ludwig-Maximilians-Universität (LMU), Geschwister-Scholl-Platz 1, 80539, Munich, Germany. tim.liedl@physik.lmu.de.
Jazyk: angličtina
Zdroj: Chembiochem : a European journal of chemical biology [Chembiochem] 2016 Jun 16; Vol. 17 (12), pp. 1093-6. Date of Electronic Publication: 2016 May 06.
DOI: 10.1002/cbic.201600059
Abstrakt: The application of commonly used force spectroscopy in biological systems is often limited by the need for an invasive tether connecting the molecules of interest to a bead or cantilever tip. Here we present a DNA origami-based prototype in a comparative binding assay. It has the advantage of in situ readout without any physical connection to the macroscopic world. The seesaw-like structure has a lever that is able to move freely relative to its base. Binding partners on each side force the structure into discrete and distinguishable conformations. Model experiments with competing DNA hybridisation reactions yielded a drastic shift towards the conformation with the stronger binding interaction. With reference DNA duplexes of tuneable length on one side, this device can be used to measure ligand interactions in comparative assays.
(© 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)
Databáze: MEDLINE
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