Creatinine Deiminase Adsorption onto Silicalite-Modified pH-FET for Creation of New Creatinine-Sensitive Biosensor.

Autor: Marchenko SV; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680, Kyiv, Ukraine., Soldatkin OO; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680, Kyiv, Ukraine. alex_sold@yahoo.com.; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska St., Kyiv, 01003, Ukraine. alex_sold@yahoo.com., Kasap BO; Micro and Nanotechnology Department, Middle East Technical University, Ankara, 06531, Turkey., Kurc BA; Micro and Nanotechnology Department, Middle East Technical University, Ankara, 06531, Turkey.; Central Laboratory, Middle East Technical University, Ankara, 06531, Turkey., Soldatkin AP; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680, Kyiv, Ukraine.; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska St., Kyiv, 01003, Ukraine., Dzyadevych SV; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03680, Kyiv, Ukraine.; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrska St., Kyiv, 01003, Ukraine.
Jazyk: angličtina
Zdroj: Nanoscale research letters [Nanoscale Res Lett] 2016 Dec; Vol. 11 (1), pp. 173. Date of Electronic Publication: 2016 Mar 31.
DOI: 10.1186/s11671-016-1386-9
Abstrakt: In the work, silicalite particles were used for the surface modification of pH-sensitive field-effect transistors (pH-FETs) with the purpose of developing new creatinine-sensitive biosensor. Creatinine deiminase (CD) adsorbed on the surface of silicalite-coated pH-FET served as a bioselective membrane. The biosensor based on CD immobilized in glutaraldehyde vapor (GA) was taken as control. The creatinine-sensitive biosensor obtained by adsorption on silicalite was shown to have better analytical characteristics (two- to threefold increased sensitivity to creatinine, three- to fourfold lesser response and recovery times, a decrease of the detection limit of creatinine determination to 5 μM, etc.).Additionally, the biosensor based on CD adsorbed on silicalite (Sil/CD) was characterized by high signal reproducibility (relative standard deviation (RSD) for creatinine measurement = 2.6 %) and stability during storage (over 13 months). It was experimentally confirmed that the proposed biosensor was not sensitive either to high concentrations of sodium chloride or to the macromolecular protein fractions and can be used for direct quantitative analysis of creatinine in the blood serum.It was concluded that the method of CD adsorption on silicalite is well-suited for the creation of creatinine-sensitive biosensor with improved working characteristics.
Databáze: MEDLINE
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