Fast production of microfluidic devices by CO2 laser engraving of wax-coated glass slides.

Autor: da Costa ET; Department of Chemistry, 219 Hunter Laboratories, Clemson University, Clemson, SC, USA., Santos MSF; Department of Chemistry, 219 Hunter Laboratories, Clemson University, Clemson, SC, USA.; Instituto de Química, Universidade de São Paulo, São Paulo, Brazil., Jiao H; HJ Science and Technology, Berkeley, CA, USA., do Lago CL; Instituto de Química, Universidade de São Paulo, São Paulo, Brazil., Gutz IG; Instituto de Química, Universidade de São Paulo, São Paulo, Brazil., Garcia CD; Department of Chemistry, 219 Hunter Laboratories, Clemson University, Clemson, SC, USA.
Jazyk: angličtina
Zdroj: Electrophoresis [Electrophoresis] 2016 Jul; Vol. 37 (12), pp. 1691-5. Date of Electronic Publication: 2016 Apr 26.
DOI: 10.1002/elps.201600065
Abstrakt: Glass is one of the most convenient materials for the development of microfluidic devices. However, most fabrication protocols require long processing times and expensive facilities. As a convenient alternative, polymeric materials have been extensively used due their lower cost and versatility. Although CO2 laser ablation has been used for fast prototyping on polymeric materials, it cannot be applied to glass devices because the local heating causes thermal stress and results in extensive cracking. A few papers have shown the ablation of channels or thin holes (used as reservoirs) on glass but the process is still far away from yielding functional glass microfluidic devices. To address these shortcomings, this communication describes a simple method to engrave glass-based capillary electrophoresis devices using standard (1 mm-thick) microscope glass slides. The process uses a sacrificial layer of wax as heat sink and enables the development of both channels (with semicircular shape) and pass-through reservoirs. Although microscope images showed some small cracks around the channels (that became irrelevant after sealing the engraved glass layer to PDMS) the proposed strategy is a leap forward in the application of the technology to glass. In order to demonstrate the capabilities of the approach, the separation of dopamine, catechol and uric acid was accomplished in less than 100 s.
(© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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