| Autor: |
Bin LL; Programa de Pós-Graduação em Ciência Animal, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil., Santarém VA; Programa de Pós-Graduação em Ciência Animal, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil., Laposy CB; Programa de Pós-Graduação em Ciência Animal, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil., Rubinsky-Elefant G; Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, SP, Brasil., Roldán WH; Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, SP, Brasil., Giuffrida R; Programa de Pós-Graduação em Ciência Animal, Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil. |
| Abstrakt: |
An evaluation was made of the kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with embryonated Toxocara canis eggs. Seventeen four month old New Zealand White rabbits were distributed into two groups. In the experimental group, twelve rabbits were infected orally with 1,000 embryonated T. canis eggs. A second group (n = 5), uninfected, was used as a control. Serum samples were collected for analysis on days 7, 14, 21, 28 and 60 post-infection (DPI). An indirect ELISA test was performed to evaluate the reactivity index (RI) of IgG anti-T. canis antibodies and to calculate the avidity index (AI). The animals showed seroconversion from the 14th DPI, with high AI (over 50%) except for one animal, which presented an intermediate AI. At 60 DPI, all the animals were seropositive and maintained a high AI. The data indicated that specific IgG antibodies formed early (14 DPI) in rabbits infected with T. canis, with a high avidity index that persisted throughout the course of the infection. |
