Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers.

Autor:	Labouesse C; Laboratory of Cell Biophysics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland., Gabella C; Laboratory of Cell Biophysics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland., Meister JJ; Laboratory of Cell Biophysics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland., Vianay B; Laboratory of Cell Biophysics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland., Verkhovsky AB; Laboratory of Cell Biophysics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Jazyk:	angličtina
Zdroj:	Scientific reports [Sci Rep] 2016 Mar 30; Vol. 6, pp. 23722. Date of Electronic Publication: 2016 Mar 30.
DOI:	10.1038/srep23722
Abstrakt:	Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through a microsurgically generated opening in the cytoplasm. By applying large deformations with a soft cantilever we were able to fully characterize the mechanical response of the fibers and evaluate their tension, extensibility, elastic and viscous properties.
Databáze:	MEDLINE
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