One-pot synthesis of a gold nanoparticle-Vmh2 hydrophobin nanobiocomplex for glucose monitoring.

Autor:	Politi J; Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, F-75005 Paris, France.; Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, Via P. Castellino 111, 80127, Italy.; Department of Chemical Sciences, University of Naples «Federico II», Via Cinthia, 80126 Naples, Italy., De Stefano L; Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, Via P. Castellino 111, 80127, Italy., Rea I; Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, Via P. Castellino 111, 80127, Italy., Gravagnuolo AM; Department of Chemical Sciences, University of Naples «Federico II», Via Cinthia, 80126 Naples, Italy., Giardina P; Department of Chemical Sciences, University of Naples «Federico II», Via Cinthia, 80126 Naples, Italy., Methivier C; Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, F-75005 Paris, France., Casale S; Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, F-75005 Paris, France.; CNRS, UMR 7244, CSPBAT, Laboratoire de Chimie, Structures et Propriétés de Biomateriaux et d'Agents Therapeutiques Université Paris 13, Sorbonne Paris Cité, Bobigny, France CNRS, Paris, France., Spadavecchia J; Sorbonne Universités, UPMC Paris 06, Laboratoire de Réactivité de Surface, 4 place Jussieu, F-75005 Paris, France.; CNRS, UMR 7244, CSPBAT, Laboratoire de Chimie, Structures et Propriétés de Biomateriaux et d'Agents Therapeutiques Université Paris 13, Sorbonne Paris Cité, Bobigny, France CNRS, Paris, France.
Jazyk:	angličtina
Zdroj:	Nanotechnology [Nanotechnology] 2016 May 13; Vol. 27 (19), pp. 195701. Date of Electronic Publication: 2016 Mar 29.
DOI:	10.1088/0957-4484/27/19/195701
Abstrakt:	HydrophobinVmh2 is a small amphiphilic protein, which self-assembles on different surfaces and naturally interacts with glucose. Here, we report on the synthesis of a nanobiocomplex made of polyethylene glycol, Vmh2 and gold nanoparticles by a one-step process and on its ability to recognise glucose in an aqueous solution at 0.3-0.6-1.2 mg ml(-1) concentrations. Even though the Vmh2 proteins are intrinsically bonded to the gold core, effective glucose interaction monitoring was demonstrated by using dynamic light scattering, ultraviolet-visible, polarization-modulated infrared reflection-absorption and x-ray photoelectron spectroscopies. Experimental results highlighted an affinity constant of 7.3 ± 0.3 mg ml(-1) between the nanobiosystem and the sugar, and a detection sensitivity of 0.13 ± 0.06 a.u./mg ml(-1).
Databáze:	MEDLINE
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