Cardioprotective Effect of Modified Peroxiredoxins in Retrograde Perfusion of Isolated Rat Heart under Conditions of Oxidative Stress.

Autor: Karaduleva EV; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia. e.v.karaduleva@gmail.com., Mubarakshina EK; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia., Sharapov MG; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia., Volkova AE; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia., Pimenov OY; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia., Ravin VK; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia., Kokoz YM; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia., Novoselov VI; Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2016 Mar; Vol. 160 (5), pp. 639-42. Date of Electronic Publication: 2016 Mar 29.
DOI: 10.1007/s10517-016-3237-1
Abstrakt: Antioxidant properties of recombinant peroxiredoxin-6 and chimeric protein PSH combining peroxidase and superoxide dismutase activities were studied on the model of retrograde perfusion of isolated rat heart under conditions of H2O2-induced oxidative stress. The exogenous antioxidant proteins exhibited cardioprotective properties manifested in heart rate normalization, maintenance of contractile activity of the myocardium, and prevention of H2O2-induced LPO in oxidative stress. Localization of peroxiredoxin-6 and PSH in the cardiac tissue was determined and myocardial structures most effectively protected by the antioxidant enzymes from ischemia/reperfusion-induced damages were identified. The results suggest that modified peroxiredoxins are promising components of perfusion media for preservation of isolated organs.
Databáze: MEDLINE
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