Detection and Characteristics of Rifampicin-Resistant Isolates of Mycobacterium tuberculosis.

Autor: Cherednichenko AG; Research Institute of Tuberculosis, Ministry of Health of the Russian Federation, Novosibirsk, Russia., Dymova MA; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. maya.a.rot@gmail.com.; Novosibirsk State University, Novosibirsk, Russia. maya.a.rot@gmail.com., Solodilova OA; Altai Regional Antituberculous Dispensary, Barnaul, Russia., Petrenko TI; Research Institute of Tuberculosis, Ministry of Health of the Russian Federation, Novosibirsk, Russia., Prozorov AI; Altai Regional Antituberculous Dispensary, Barnaul, Russia., Filipenko ML; Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.; Novosibirsk State University, Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2016 Mar; Vol. 160 (5), pp. 659-63. Date of Electronic Publication: 2016 Mar 29.
DOI: 10.1007/s10517-016-3243-3
Abstrakt: Genotyping and analysis the drug resistance of 59 isolates of M. tuberculosis obtained from patients living in Altai Territory were performed using a BACTEC MGIT 960 fluorometric system by means of VNTR typing (variable number tandem repeat), PCR-RFLP analysis, and sequence analysis. The occurrence frequency was highest for isolates of the Beijing family (n=30, 50.8%). Analysis of mutation spectrum in the rpoB gene associated with rifampicin resistance revealed the major mutation (codon 531 of the rpoB gene) in 93% samples, which allows us to use rapid test systems.
Databáze: MEDLINE
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