Molecular analysis of hemagglutinin-1 fragment of avian influenza H5N1 viruses isolated from chicken farms in Indonesia from 2008 to 2010.

Autor: Mahardika GN; Animal Biomedical and Molecular Biology Laboratory, Udayana University, Sesetan-Markisa Street No. 6, Denpasar 80226, Bali, Indonesia. Electronic address: gnmahardika@indosat.net.id., Jonas M; Research and Development Department, PT. Medion, Babakan Ciparay Street #282, Bandung, Indonesia., Murwijati T; Research and Development Department, PT. Medion, Babakan Ciparay Street #282, Bandung, Indonesia., Fitria N; Research and Development Department, PT. Medion, Babakan Ciparay Street #282, Bandung, Indonesia., Suartha IN; Animal Biomedical and Molecular Biology Laboratory, Udayana University, Sesetan-Markisa Street No. 6, Denpasar 80226, Bali, Indonesia., Suartini IG; Animal Biomedical and Molecular Biology Laboratory, Udayana University, Sesetan-Markisa Street No. 6, Denpasar 80226, Bali, Indonesia., Wibawan IW; Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, West Java, Indonesia.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2016 Apr 15; Vol. 186, pp. 52-8. Date of Electronic Publication: 2016 Feb 26.
DOI: 10.1016/j.vetmic.2016.02.023
Abstrakt: Highly pathogenic avian influenza virus of subtype H5N1 (AIV-H5N1) has been circulating in Indonesia since 2003. To understand the genetic diversity of these viruses, and to predict vaccine efficacy, the hemaglutinin-1 (HA-1) fragment of viruses isolated from chicken farms in Indonesia from 2008 to 2010 was sequenced and analyzed. The effects of these molecular changes were investigated in challenge experiments and HI assays of homologous and heterologous strains. Molecular analysis showed that these AIV-H5N1 isolates had evolved into three distinct sub-lineages from an ancestor circulating since 2003. Although no significant positive selection of residues was detected, 12 negatively selected sites were identified (p<0.05). Moreover, four sites showed evidence of significant episodic diversifying selection. The findings indicated complete protectivity and high HI titers with homologous strains, compared with protectivity ranging from 40 to 100% and lower HI titers with heterologous strains resulting from polymorphisms at antigenic sites. Our findings provide valuable insight into the molecular evolution of AIV and have important implications for vaccine efficacy and future vaccination strategies.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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