| Autor: |
Tsen SD; Department of Radiology, Washington University School of Medicine, St Louis, MO 63110., Kibler K; Biodesign Institute, Arizona State University, Tempe, AZ 85287., Jacobs B; Biodesign Institute, Arizona State University, Tempe, AZ 85287., Fay JC; Department of Genetics, Washington University School of Medicine, St Louis, MO 63110., Podolnikova NP; ASU/Mayo Center for Metabolic and Vascular Biology, Arizona State University Tempe, AZ 85287., Ugarova TP; ASU/Mayo Center for Metabolic and Vascular Biology, Arizona State University Tempe, AZ 85287., Achilefu S; Department of Radiology, Washington University School of Medicine, St Louis, MO 63110., Tsen KT; Department of Physics and Center for Biophysics, Arizona State University, Tempe, AZ 85287-1504. |
| Abstrakt: |
Microbial contamination of cell culture is a major problem encountered both in academic labs and in the biotechnology/pharmaceutical industries. A broad spectrum of microbes including mycoplasma, bacteria, fungi, and viruses are the causative agents of cell culture contamination. Unfortunately, the existing disinfection techniques lack selectivity and/or lead to the development of drug-resistance, and more importantly there is no universal method to address all microbes. Here, we report a novel, chemical-free visible ultrashort pulsed laser method for cell culture disinfection. The ultrashort pulsed laser technology inactivates pathogens with mechanical means, a paradigm shift from the traditional pharmaceutical and chemical approaches. We demonstrate that ultrashort pulsed laser treatment can efficiently inactivate mycoplasma, bacteria, yeast, and viruses with good preservation of mammalian cell viability. Our results indicate that this ultrashort pulsed laser technology has the potential to serve as a universal method for the disinfection of cell culture. |
