| Autor: |
Dana H; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Mohar B; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.; Weizmann Institute of Science, Rehovot, Israel., Sun Y; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Narayan S; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Gordus A; Howard Hughes Medical Institute, The Rockefeller University, New York, United States., Hasseman JP; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Tsegaye G; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Holt GT; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Hu A; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Walpita D; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Patel R; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Macklin JJ; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Bargmann CI; Howard Hughes Medical Institute, The Rockefeller University, New York, United States., Ahrens MB; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Schreiter ER; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Jayaraman V; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Looger LL; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Svoboda K; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Kim DS; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States. |
| Abstrakt: |
Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging. |
