Roquin recognizes a non-canonical hexaloop structure in the 3'-UTR of Ox40.

Autor: Janowski R; Group Intracellular Transport and RNA Biology, Institute of Structural Biology, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, Neuherberg DE-85764, Germany., Heinz GA; Research Unit Molecular Immune Regulation, Helmholtz Zentrum München, Marchioninistrasse 25, München DE-81377, Germany.; Institute for Immunology at the Biomedical Center, Ludwig-Maximilians-Universität München, Grosshaderner Strasse 9, Planegg-Martinsried DE-82152, Germany., Schlundt A; Institute of Structural Biology, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, Neuherberg DE-85764, Germany.; Center for Integrated Protein Science Munich at Biomolecular NMR Spectroscopy, Department Chemie, Technische Universität München, Lichtenbergstrasse 4, Garching DE-85747, Germany., Wommelsdorf N; Research Unit Molecular Immune Regulation, Helmholtz Zentrum München, Marchioninistrasse 25, München DE-81377, Germany.; Institute for Immunology at the Biomedical Center, Ludwig-Maximilians-Universität München, Grosshaderner Strasse 9, Planegg-Martinsried DE-82152, Germany., Brenner S; Research Unit Molecular Immune Regulation, Helmholtz Zentrum München, Marchioninistrasse 25, München DE-81377, Germany., Gruber AR; Biozentrum, University of Basel and Swiss Institute of Bioinformatics, Klingelbergstrasse 50-70, Basel CH-4056, Switzerland., Blank M; AptaIT GmbH, Goethestrasse 52, München 80336, Germany., Buch T; Institute of Laboratory Animal Science, University of Zurich, Wagistrasse 12, Schlieren 8952, Switzerland., Buhmann R; AptaIT GmbH, Goethestrasse 52, München 80336, Germany.; Department of Medicine III and Transfusion Medicine, University Hospital Grosshadern, LMU, Marchioninistrasse 15, München 81377, Germany., Zavolan M; Biozentrum, University of Basel and Swiss Institute of Bioinformatics, Klingelbergstrasse 50-70, Basel CH-4056, Switzerland., Niessing D; Group Intracellular Transport and RNA Biology, Institute of Structural Biology, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, Neuherberg DE-85764, Germany.; Department of Cell Biology at the Biomedical Center, Ludwig-Maximilians-Universität München, Grosshaderner Strasse 9, Planegg-Martinsried DE-82152, Germany., Heissmeyer V; Research Unit Molecular Immune Regulation, Helmholtz Zentrum München, Marchioninistrasse 25, München DE-81377, Germany.; Institute for Immunology at the Biomedical Center, Ludwig-Maximilians-Universität München, Grosshaderner Strasse 9, Planegg-Martinsried DE-82152, Germany., Sattler M; Institute of Structural Biology, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, Neuherberg DE-85764, Germany.; Center for Integrated Protein Science Munich at Biomolecular NMR Spectroscopy, Department Chemie, Technische Universität München, Lichtenbergstrasse 4, Garching DE-85747, Germany.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2016 Mar 24; Vol. 7, pp. 11032. Date of Electronic Publication: 2016 Mar 24.
DOI: 10.1038/ncomms11032
Abstrakt: The RNA-binding protein Roquin is required to prevent autoimmunity. Roquin controls T-helper cell activation and differentiation by limiting the induced expression of costimulatory receptors such as tumor necrosis factor receptor superfamily 4 (Tnfrs4 or Ox40). A constitutive decay element (CDE) with a characteristic triloop hairpin was previously shown to be recognized by Roquin. Here we use SELEX assays to identify a novel U-rich hexaloop motif, representing an alternative decay element (ADE). Crystal structures and NMR data show that the Roquin-1 ROQ domain recognizes hexaloops in the SELEX-derived ADE and in an ADE-like variant present in the Ox40 3'-UTR with identical binding modes. In cells, ADE-like and CDE-like motifs cooperate in the repression of Ox40 by Roquin. Our data reveal an unexpected recognition of hexaloop cis elements for the posttranscriptional regulation of target messenger RNAs by Roquin.
Databáze: MEDLINE
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