| Autor: |
Francisco CO; Departamento de Fisioterapia, Universidade Federal de São Carlos, São Carlos, SP, Brasil., Catai AM; Departamento de Fisioterapia, Universidade Federal de São Carlos, São Carlos, SP, Brasil., Moura-Tonello SC; Departamento de Fisioterapia, Universidade Federal de São Carlos, São Carlos, SP, Brasil., Arruda LC; Centro de Terapia Celular, Fundação de Amparo è Pesquisa do Estado de São Paulo, Ribeirão Preto, SP, Brasil., Lopes SL; Departamento de Medicina, Universidade Federal de São Carlos, São Carlos, SP, Brasil., Benze BG; Departamento de Estatística, Universidade Federal de São Carlos, São Carlos, SP, Brasil., Del Vale AM; Departamento de Medicina, Universidade Federal de São Carlos, São Carlos, SP, Brasil., Malmegrim KC; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil., Leal AM; Departamento de Medicina, Universidade Federal de São Carlos, São Carlos, SP, Brasil. |
| Abstrakt: |
Type 2 diabetes mellitus (T2D) is a metabolic disease with inflammation as an important pathogenic background. However, the pattern of immune cell subsets and the cytokine profile associated with development of T2D are unclear. The objective of this study was to evaluate different components of the immune system in T2D patients' peripheral blood by quantifying the frequency of lymphocyte subsets and intracellular pro- and anti-inflammatory cytokine production by T cells. Clinical data and blood samples were collected from 22 men (51.6±6.3 years old) with T2D and 20 nonsmoking men (49.4±7.6 years old) who were matched for age and sex as control subjects. Glycated hemoglobin, high-sensitivity C-reactive protein concentrations, and the lipid profile were measured by a commercially available automated system. Frequencies of lymphocyte subsets in peripheral blood and intracellular production of interleukin (IL)-4, IL-10, IL-17, tumor necrosis factor-α, and interferon-γ cytokines by CD3+ T cells were assessed by flow cytometry. No differences were observed in the frequency of CD19+ B cells, CD3+CD8+ and CD3+CD4+ T cells, CD16+56+ NK cells, and CD4+CD25+Foxp3+ T regulatory cells in patients with T2D compared with controls. The numbers of IL-10- and IL-17-producing CD3+ T cells were significantly higher in patients with T2D than in controls (P<0.05). The frequency of interferon-γ-producing CD3+ T cells was positively correlated with body mass index (r=0.59; P=0.01). In conclusion, this study shows increased numbers of circulating IL-10- and IL-17-producing CD3+ T cells in patients with T2D, suggesting that these cytokines are involved in the immune pathology of this disease. |
