| Autor: |
Gasulla J; Instituto de Microbiología y Zoología Agrícola (IMyZA), Instituto Nacional de Tecnología Agropecuaria (INTA) - CONICET, Las Cabañas y De los Reseros s/n, Hurlingham, Buenos Aires, Argentina., Picco SJ; Instituto de Genética Veterinaria 'Fernando Noel Dulout' (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata - CONICET, Calle 60 y 117 (1900), La Plata, Buenos Aires, Argentina., Carriquiriborde P; Centro de Investigaciones del Medio Ambiente (CIMA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata - CONICET, Calle 47 y 115 s/n (1900), La Plata, Buenos Aires, Argentina. pcarriquiriborde@quimica.unlp.edu.ar., Dulout FN; Instituto de Genética Veterinaria 'Fernando Noel Dulout' (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata - CONICET, Calle 60 y 117 (1900), La Plata, Buenos Aires, Argentina., Ronco AE; Centro de Investigaciones del Medio Ambiente (CIMA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata - CONICET, Calle 47 y 115 s/n (1900), La Plata, Buenos Aires, Argentina., de Luca JC; Instituto de Genética Veterinaria 'Fernando Noel Dulout' (IGEVET), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata - CONICET, Calle 60 y 117 (1900), La Plata, Buenos Aires, Argentina. |
| Abstrakt: |
Genotoxic effects of Cd(+2), Cr(+6), and Cu(+2) on the gill and liver of the Argentinean Silverside (Odontesthes bonariensis) were studied using the comet assay and in relation with the metal tissue accumulation. Fish were exposed to three waterborne concentrations of each metal for 2 and 16 days. Genotoxicity was assessed by the single cell gel electrophoresis (comet assay). After 2 days, significant increase of the genetic damage index (GDI) was only observed in the gill of fish exposed to Cr(+6) and Cu(+2), and the LOECs were 2160 nM and 921.1 nM, respectively. The gill LOEC for Cd(+2) by 16 days was 9.4 nM. In the liver, LOECs were obtained only for Cd(+2) and Cr(+6) and were 9.4 and 2160 nM, respectively. The three metals were able to induce genotoxic effects at environmentally relevant concentrations and the gill was the most sensitive organ. |
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