Further studies on the cytoskeleton of resident, stimulated and activated macrophages.

Autor: del Caro LM, de Souza MF, de Souza W
Jazyk: angličtina
Zdroj: Microscopia electronica y biologia celular : organo oficial de las Sociedades Latinoamericana de Microscopia Electronica e Iberoamericana de Biologia Celular [Microsc Electron Biol Celular] 1989 Jun; Vol. 13 (1), pp. 1-17.
Abstrakt: The three-dimensional organization of the cytoskeleton of resident, thioglycolate-elicited and activated macrophages was studied by indirect immunofluorescence microscopy and by transmission electron microscopy of platinum-carbon replicas of Triton X-100 extracted cells and whole extracted cells critical point dried. Examination of the replicas showed with great clarity the organization of cytoplasmic filaments and microtubules. Cytoskeletal preparations revealed filamentous structures around the centrioles, and dense granules dispersed within the lattice of filaments of activated macrophages. X-ray microanalysis showed that they concentrate osmium. Indirect immunofluorescence microscopy revealed filamentous and/or dispersed tubulin, actin, vimentin and myosin-containing structures in the cytoplasm of the macrophages.
Databáze: MEDLINE