Repeat-based Sequence Typing of Carnobacterium maltaromaticum.

Autor: Rahman A; Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules (LIBio), ENSAIA, 2 avenue de la Forêt de Haye, TSA, 40602 54518 Vandœuvre-lès-Nancy, France; National University of Sciences and Technology, Atta ur Rahman School of Applied Biosciences, Department of Industrial Biotechnology, 44000, H-12, Islamabad, Pakistan., El Kheir SM; Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules (LIBio), ENSAIA, 2 avenue de la Forêt de Haye, TSA, 40602 54518 Vandœuvre-lès-Nancy, France., Back A; Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules (LIBio), ENSAIA, 2 avenue de la Forêt de Haye, TSA, 40602 54518 Vandœuvre-lès-Nancy, France., Mangavel C; Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules (LIBio), ENSAIA, 2 avenue de la Forêt de Haye, TSA, 40602 54518 Vandœuvre-lès-Nancy, France., Revol-Junelles AM; Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules (LIBio), ENSAIA, 2 avenue de la Forêt de Haye, TSA, 40602 54518 Vandœuvre-lès-Nancy, France., Borges F; Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules (LIBio), ENSAIA, 2 avenue de la Forêt de Haye, TSA, 40602 54518 Vandœuvre-lès-Nancy, France. Electronic address: frederic.borges@univ-lorraine.fr.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2016 Jun 02; Vol. 226, pp. 1-4. Date of Electronic Publication: 2016 Mar 04.
DOI: 10.1016/j.ijfoodmicro.2016.03.003
Abstrakt: Carnobacterium maltaromaticum is a Lactic Acid Bacterium (LAB) of technological interest for the food industry, especially the dairy as bioprotection and ripening flora. The industrial use of this LAB requires accurate and resolutive typing tools. A new typing method for C. maltaromaticum inspired from MLVA analysis and called Repeat-based Sequence Typing (RST) is described. Rather than electrophoresis analysis, our RST method is based on sequence analysis of multiple loci containing Variable-Number Tandem-Repeats (VNTRs). The method described here for C. maltaromaticum relies on the analysis of three VNTR loci, and was applied to a collection of 24 strains. For each strain, a PCR product corresponding to the amplification of each VNTR loci was sequenced. Sequence analysis allowed delineating 11, 11, and 12 alleles for loci VNTR-A, VNTR-B, and VNTR-C, respectively. Considering the allele combination exhibited by each strain allowed defining 15 genotypes, ending in a discriminatory index of 0.94. Comparison with MLST revealed that both methods were complementary for strain typing in C. maltaromaticum.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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