Comparison of three methods for the detection of Angiostrongylus vasorum in the final host.

Autor: Houpin E; Ecole Nationale Vétérinaire de Toulouse (ENVT), France. Electronic address: houpin.emilie@gmail.com., McCarthy G; School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland., Ferrand M; Department de Genie Biologique, Institut Universitaire de Technologie, Universite Claude Bernard, Lyon, France. Electronic address: maxence.ferrand@etu.univ-lyon1.fr., De Waal T; School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland., O'Neill EJ; School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland., Zintl A; School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland. Electronic address: annetta.zintl@ucd.ie.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2016 Apr 15; Vol. 220, pp. 54-8. Date of Electronic Publication: 2016 Feb 27.
DOI: 10.1016/j.vetpar.2016.02.023
Abstrakt: Angiostrongylosis is potentially fatal parasitic nematode infection affecting dogs which can be difficult to diagnose. In recent years several microscopical, serological and molecular detection methods have been developed, however there are few studies that have compared the relative performance of these methods. Screening necropsy material from an opportunistic sample of 140 foxes (82 of which were considered to be infected with Angiostrongylus vasorum), indicated sensitivities of 84.1% for dissection and visual examination of plucks, 69.5% for nested PCR of an 18S rRNA fragment and 76.8% for a canine A. vasorum antigen detection test (IDEXX Angio Detect) of tissue fluid samples respectively. Agreement between the tests ranged from 45.6 to 79.7%. A novel nested PCR-RFLP for the detection and identification of canid lungworm spp. is described.
(Copyright © 2016 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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