Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability.

Autor:	Fonseca MI; Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina. Electronic address: biotecmol2010@gmail.com., Tejerina MR; Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina., Sawostjanik-Afanasiuk SS; Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina., Giorgio EM; Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina., Barchuk ML; Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina., Zapata PD; Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina., Villalba LL; Laboratorio de Biotecnología Molecular, Módulo de Bioquímica y Farmacia, Facultad de Ciencias Exactas Químicas y Naturales, UNaM, Posadas, Misiones, Argentina.
Jazyk:	angličtina
Zdroj:	Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2016 Apr-Jun; Vol. 47 (2), pp. 287-97. Date of Electronic Publication: 2016 Mar 02.
DOI:	10.1016/j.bjm.2016.01.002
Abstrakt:	Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined. The selected strains produced variable amounts of laccase and MnP; when Cu(2+) was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes were increased in all strains in the presence of Cu(2+). Strain B showed the greatest response to Cu(2+) addition, whereas strain A was more stable at the optimal temperature and pH. Strain A showed interesting potential for future biotechnological approaches due to the superior thermo-stability of its secreted enzymes. (Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.)
Databáze:	MEDLINE
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