Expression of TGF-β3 in isolated fibroblasts from foreskin.
| Autor: | Mahmoudi Rad M; Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Mahmoudi Rad N; Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Mirdamadi Y; Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Department of Dermatology and Venereology, Otto-von Guericke University Magdeburg, Magdeburg, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | Reports of biochemistry & molecular biology [Rep Biochem Mol Biol] 2015 Apr; Vol. 3 (2), pp. 76-81. |
| Abstrakt: | Background: The multifunctional transforming growth factor beta (TGF-β) is a glycoprotein that exists in three isoforms. TGF-β3 expression increases in fetal wound healing and reduces fibronectin and collagen I and III deposition, and also improves the architecture of the neodermis which is a combination of blood vessels and connective tissue during wound healing. Fibroblasts are key cells in the wound healing process. TGF-β3 plays a critical role in scar-free wound healing and fibroblast actions in the wound healing process. The aim of this study was to express the TGF-β3 gene (tgf-b3) in human foreskin fibroblasts (HFF's). Methods: We obtained HFF's from a newborn and a primary fibroblast culture was prepared. The cells were transfected with TGF-β3-pCMV6-XL5 plasmid DNA by both lipofection and electroporation. Expression of TGF-β3 was measured by enzyme-linked immunosorbent assay (ELISA). Results: The highest TGF-β3 expression (8.3-fold greater than control) was obtained by lipofection after 72 hours using 3 µl of transfection reagent. Expression was 1.4-fold greater than control by electroporation. Conclusions: In this study, we successfully increased TGF-β3 expression in primary fibroblast cells. In the future, grafting these transfected fibroblasts onto wounds can help the healing process without scarring. |
| Databáze: | MEDLINE |
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