Therapeutic and Safety Evaluation of Combined Aqueous Extracts of Azadirachta indica and Khaya senegalensis in Chickens Experimentally Infected with Eimeria Oocysts.

Autor:	Gotep JG; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Tanko JT; Parasitology Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Forcados GE; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Muraina IA; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Ozele N; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Dogonyaro BB; Virology Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Oladipo OO; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Makoshi MS; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Akanbi OB; Central Diagnostics Laboratory, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Kinjir H; Haematology Department, Federal College of Veterinary and Medical Laboratory Technology, PMB 01, Vom, Nigeria., Samuel AL; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Onyiche TE; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria; Department of Veterinary Microbiology and Parasitology, University of Maiduguri, Bama Road, Maiduguri, Borno State, Nigeria., Ochigbo GO; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria; Department of Veterinary Physiology, Pharmacology and Biochemistry, University of Ibadan, PMB 0248, Ibadan, Nigeria., Aladelokun OB; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria; Department of Biochemistry, University of Ibadan, PMB 0248, Ibadan, Nigeria., Ozoani HA; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria; Department of Medical Laboratory Science, Rivers State University of Science and Technology, PMB 5080, Port Harcourt, Nigeria., Viyoff VZ; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria; Department of Epidemiology, University of Buea, P.O. Box 63, Buea, Cameroon., Dapuliga CC; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria; Microbiology Department, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana., Atiku AA; Biochemistry Division, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Okewole PA; Central Diagnostics Laboratory, National Veterinary Research Institute, PMB 01, Vom, Nigeria., Shamaki D; National Veterinary Research Institute, Vom, Nigeria., Ahmed MS; National Veterinary Research Institute, Vom, Nigeria., Nduaka CI; Africa Education Initiative (NEF), 9401 Sentinel Ridge, Eagleville, PA 19403, USA.
Jazyk:	angličtina
Zdroj:	Journal of parasitology research [J Parasitol Res] 2016; Vol. 2016, pp. 4692424. Date of Electronic Publication: 2016 Feb 16.
DOI:	10.1155/2016/4692424
Abstrakt:	Coccidiosis is a disease of economic importance in poultry causing morbidity and mortality. Reports show that Azadirachta indica and Khaya senegalensis have been used individually in the treatment of avian coccidiosis. We thus investigated the efficacy and safety of the combined aqueous extracts of these plants for the treatment of experimentally induced coccidiosis in broiler chickens using oocyst count, oxidative stress biomarkers, serum biochemistry, histology, and haematological parameters. The phytochemical screening revealed the presence of tannins, saponins, cardiac glycosides, and steroids in both extracts. In addition, alkaloids and flavonoids were present in Azadirachta indica. There was significant (p < 0.05) dose dependent decrease in oocyst count across the treatment groups with 400 mg/kg of the combined extract being the most efficacious dose. Immunomodulatory and erythropoietic activity was observed. There were decreased intestinal lesions and enhanced antioxidant activity across the treatment groups compared to the negative control. Administration of the combined extract did not cause damage to the liver as ALT, AST, and ALP levels were significantly reduced in the uninfected chickens treated with the extracts compared to control suggesting safety at the doses used. The combined aqueous extracts of K. senegalensis stem bark and Azadirachta indica leaves were ameliorative in chickens infected with coccidiosis.
Databáze:	MEDLINE
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