Genome-wide Screening Identifies Phosphotransferase System Permease BepA to Be Involved in Enterococcus faecium Endocarditis and Biofilm Formation.

Autor:	Paganelli FL; Department of Medical Microbiology, University Medical Center Utrecht, The Netherlands., Huebner J; Division of Pediatric Infectious Diseases, Hauner Children's Hospital, Ludwigs-Maximilian Universität München Center for Infectious Disease and Travel Medicine, University Medical Center Freiburg, Germany., Singh KV; Department of Internal Medicine, Division of Infectious Diseases Center for the Study of Emerging and Re-emerging Pathogens., Zhang X; Department of Medical Microbiology, University Medical Center Utrecht, The Netherlands., van Schaik W; Department of Medical Microbiology, University Medical Center Utrecht, The Netherlands., Wobser D; Center for Infectious Disease and Travel Medicine, University Medical Center Freiburg, Germany., Braat JC; Department of Medical Microbiology, University Medical Center Utrecht, The Netherlands., Murray BE; Department of Internal Medicine, Division of Infectious Diseases Center for the Study of Emerging and Re-emerging Pathogens Department of Microbiology and Molecular Genetics, University of Texas Health Science Center at Houston., Bonten MJ; Department of Medical Microbiology, University Medical Center Utrecht, The Netherlands., Willems RJ; Department of Medical Microbiology, University Medical Center Utrecht, The Netherlands., Leavis HL; Department of Medical Microbiology, University Medical Center Utrecht, The Netherlands.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2016 Jul 15; Vol. 214 (2), pp. 189-95. Date of Electronic Publication: 2016 Mar 16.
DOI:	10.1093/infdis/jiw108
Abstrakt:	Enterococcus faecium is a common cause of nosocomial infections, of which infective endocarditis is associated with substantial mortality. In this study, we used a microarray-based transposon mapping (M-TraM) approach to evaluate a rat endocarditis model and identified a gene, originally annotated as "fruA" and renamed "bepA," putatively encoding a carbohydrate phosphotransferase system (PTS) permease (biofilm and endocarditis-associated permease A [BepA]), as important in infective endocarditis. This gene is highly enriched in E. faecium clinical isolates and absent in commensal isolates that are not associated with infection. Confirmation of the phenotype was established in a competition experiment of wild-type and a markerless bepA mutant in a rat endocarditis model. In addition, deletion of bepA impaired biofilm formation in vitro in the presence of 100% human serum and metabolism of β-methyl-D-glucoside. β-glucoside metabolism has been linked to the metabolism of glycosaminoglycans that are exposed on injured heart valves, where bacteria attach and form vegetations. Therefore, we propose that the PTS permease BepA is directly implicated in E. faecium pathogenesis. (© The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.)
Databáze:	MEDLINE
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