Microscopy tools for the investigation of intracellular lipid storage and dynamics.

Autor:	Daemen S; Department of Human Movement Sciences and Human Biology, NUTRIM School for Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands. Electronic address: sabine.daemen@maastrichtuniversity.nl., van Zandvoort MAMJ; Department of Genetics and Molecular Cell Biology, CARIM School for Cardiovascular Diseases, Maastricht University, Maastricht, The Netherlands; Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, Aachen, Germany. Electronic address: mamj.vanzandvoort@maastrichtuniversity.nl., Parekh SH; Department of Molecular Spectroscopy, Max Planck Institute for Polymer Research, Mainz, Germany. Electronic address: parekh@mpip-mainz.mpg.de., Hesselink MKC; Department of Human Movement Sciences and Human Biology, NUTRIM School for Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands. Electronic address: matthijs.hesselink@maastrichtuniversity.nl.
Jazyk:	angličtina
Zdroj:	Molecular metabolism [Mol Metab] 2015 Dec 31; Vol. 5 (3), pp. 153-163. Date of Electronic Publication: 2015 Dec 31 (Print Publication: 2016).
DOI:	10.1016/j.molmet.2015.12.005
Abstrakt:	Background: Excess storage of lipids in ectopic tissues, such as skeletal muscle, liver, and heart, seems to associate closely with metabolic abnormalities and cardiac disease. Intracellular lipid storage occurs in lipid droplets, which have gained attention as active organelles in cellular metabolism. Recent developments in high-resolution microscopy and microscopic spectroscopy have opened up new avenues to examine the physiology and biochemistry of intracellular lipids. Scope of Review: The aim of this review is to give an overview of recent technical advances in microscopy, and its application for the visualization, identification, and quantification of intracellular lipids, with special focus to lipid droplets. In addition, we attempt to summarize the probes currently available for the visualization of lipids. Major Conclusions: The continuous development of lipid probes in combination with the rapid development of microscopic techniques can provide new insights in the role and dynamics of intracellular lipids. Moreover, in situ identification of intracellular lipids is now possible and promises to add a new dimensionality to analysis of lipid biochemistry, and its relation to (patho)physiology.
Databáze:	MEDLINE
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