Human cytomegalovirus-encoded US28 may act as a tumor promoter in colorectal cancer.

Autor:	Cai ZZ; Zhen-Zhai Cai, Yu-Hui Zhou, Shu-Zhi Zheng, Meng-Si Ye, Yun He, Zhan-Xiong Xue, Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China., Xu JG; Zhen-Zhai Cai, Yu-Hui Zhou, Shu-Zhi Zheng, Meng-Si Ye, Yun He, Zhan-Xiong Xue, Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China., Zhou YH; Zhen-Zhai Cai, Yu-Hui Zhou, Shu-Zhi Zheng, Meng-Si Ye, Yun He, Zhan-Xiong Xue, Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China., Zheng JH; Zhen-Zhai Cai, Yu-Hui Zhou, Shu-Zhi Zheng, Meng-Si Ye, Yun He, Zhan-Xiong Xue, Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China., Lin KZ; Zhen-Zhai Cai, Yu-Hui Zhou, Shu-Zhi Zheng, Meng-Si Ye, Yun He, Zhan-Xiong Xue, Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China., Zheng SZ; Zhen-Zhai Cai, Yu-Hui Zhou, Shu-Zhi Zheng, Meng-Si Ye, Yun He, Zhan-Xiong Xue, Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China., Ye MS; Zhen-Zhai Cai, Yu-Hui Zhou, Shu-Zhi Zheng, Meng-Si Ye, Yun He, Zhan-Xiong Xue, Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China., He Y; Zhen-Zhai Cai, Yu-Hui Zhou, Shu-Zhi Zheng, Meng-Si Ye, Yun He, Zhan-Xiong Xue, Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China., Liu CB; Zhen-Zhai Cai, Yu-Hui Zhou, Shu-Zhi Zheng, Meng-Si Ye, Yun He, Zhan-Xiong Xue, Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China., Xue ZX; Zhen-Zhai Cai, Yu-Hui Zhou, Shu-Zhi Zheng, Meng-Si Ye, Yun He, Zhan-Xiong Xue, Department of Gastroenterology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China.
Jazyk:	angličtina
Zdroj:	World journal of gastroenterology [World J Gastroenterol] 2016 Mar 07; Vol. 22 (9), pp. 2789-98.
DOI:	10.3748/wjg.v22.i9.2789
Abstrakt:	Aim: To assess human cytomegalovirus-encoded US28 gene function in colorectal cancer (CRC) pathogenesis. Methods: Immunohistochemical analysis was performed to determine US28 expression in 103 CRC patient samples and 98 corresponding adjacent noncancerous samples. Patient data were compared by age, sex, tumor location, histological grade, Dukes' stage, and overall mean survival time. In addition, the US28 gene was transiently transfected into the CRC LOVO cell line, and cell proliferation was assessed using a cell counting kit-8 assay. Cell cycle analysis by flow cytometry and a cell invasion transwell assay were also carried out. Results: US28 levels were clearly higher in CRC tissues (38.8%) than in adjacent noncancerous samples (7.1%) (P = 0.000). Interestingly, elevated US28 amounts in CRC tissues were significantly associated with histological grade, metastasis, Dukes' stage, and overall survival (all P < 0.05); meanwhile, US28 expression was not significantly correlated with age, sex or tumor location. In addition, multivariate Cox regression data revealed US28 level as an independent CRC prognostic marker (P = 0.000). LOVO cells successfully transfected with the US28 gene exhibited higher viability, greater chemotherapy resistance, accelerated cell cycle progression, and increased invasion ability. Conclusion: US28 expression is predictive of poor prognosis and may promote CRC.
Databáze:	MEDLINE
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