| Autor: |
Weiss T; R-Biopharm AG, An der neuen Bergstrasse 17, D-64297 Darmstadt, Germany., Lacorn M, Flannery J, Benzinger MJ, Bird P, Crowley ES, Goins D, Agin JR, Gilani S; FAOACI, Foreign Fellow of Pakistan Academy of Science, Ottawa, Ontario, Canada., Poepping B; Merieux NutriSciences, 113 Route De Paris, 69160 Tassin La Demi Lune, France., Garber E; U.S. Food and Drug Administration, 5100 Paint Branch Pkwy, College Park, MD 20740, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of AOAC International [J AOAC Int] 2016 Mar-Apr; Vol. 99 (2), pp. 495-503. Date of Electronic Publication: 2016 Mar 08. |
| DOI: |
10.5740/jaoacint.15-0290 |
| Abstrakt: |
The RIDASCREEN(®)FAST Milk test is a sandwich ELISA for the rapid quantification of milk proteins in various foods. The specific antibodies target casein and β-lactoglobulin. Samples are extracted and can then be analyzed in less than 40 min. The calibration curve covers a range from 2.5 to 67.5 mg/kg milk protein. The assay was validated with cookies, infant formula, chocolate dessert, ice cream, and sausages. All negative samples were found well below the LOQ of 2.5 mg/kg. Recoveries of the spiked samples were mostly in the range of 80-120%. The LOD of the ELISA was found below 1 mg/kg. The analysis of 39 different substances of interest revealed that no cross-reactivity above the LOQ occurred. Ruggedness testing proved that variations in incubation temperature, reagent volume, incubation time, extraction temperature, and extraction time had no significant influence. The stability at 4-8°C of three independent lots was investigated and found to exceed 18 months. Very good lot-to-lot consistency and no significant loss of the analytical capacity over the shelf life were observed. Incurred cookies and chocolate dessert samples were prepared and analyzed by an independent laboratory; mean recoveries of 94.4 and 102.2% and mean SDs of 10.9 and 6.3%, respectively, were found. For the 0 mg/kg level for both materials, all samples tested returned values of <2.5 mg/kg. Therefore, the analytical performance claims of the manufacturer were confirmed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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