The use of fluorescently-tagged apoptolidins in cellular uptake and response studies.

Autor: Chong KM; Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA., Leelatian N; Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA., Deguire SM; Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA., Brockman AA; Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA., Earl D; Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA., Ihrie RA; Department of Neurological Surgery, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA., Irish JM; Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN, USA., Bachmann BO; Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Chemistry, Vanderbilt University, Nashville, TN, USA., Sulikowski GA; Vanderbilt Institute of Chemical Biology, Vanderbilt University Medical Center, Nashville, TN, USA.; Department of Chemistry, Vanderbilt University, Nashville, TN, USA.
Jazyk: angličtina
Zdroj: The Journal of antibiotics [J Antibiot (Tokyo)] 2016 Apr; Vol. 69 (4), pp. 327-30. Date of Electronic Publication: 2016 Mar 09.
DOI: 10.1038/ja.2016.22
Abstrakt: The apoptolidins are glycomacrolide microbial metabolites reported to be selectively cytotoxic against tumor cells. Using fluorescently tagged active derivatives we demonstrate selective uptake of these four tagged glycomacrolides in cancer cells over healthy human blood cells. We also demonstrate the utility of these five fluorescently tagged glycomacrolides in fluorescent flow cytometry to monitor cellular uptake of the six glycomacrolides and cellular response.
Databáze: MEDLINE
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