Simultaneous Determination of Potassium Sorbate, Sodium Benzoate, Quinoline Yellow and Sunset Yellow in Lemonades and Lemon Sauces by HPLC Using Experimental Design.
| Autor: | Dinç Zor Ş; Department of Chemistry, Faculty of Science and Arts, Yıldız Technical University, Istanbul, Turkey sule_dinc@yahoo.com., Aşçı B; Department of Chemistry, Faculty of Science and Arts, Yıldız Technical University, Istanbul, Turkey., Aksu Dönmez Ö; Department of Chemistry, Faculty of Science and Arts, Yıldız Technical University, Istanbul, Turkey., Yıldırım Küçükkaraca D; Department of Chemistry, Faculty of Science and Arts, Yıldız Technical University, Istanbul, Turkey. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of chromatographic science [J Chromatogr Sci] 2016 Jul; Vol. 54 (6), pp. 952-7. Date of Electronic Publication: 2016 Mar 06. |
| DOI: | 10.1093/chromsci/bmw027 |
| Abstrakt: | In this study, development and validation of a HPLC method was described for simultaneous determination of potassium sorbate, sodium benzoate, quinoline yellow and sunset yellow. A Box-Behnken design using three variables at three levels was employed to determine the optimum conditions of chromatographic separation: pH of mobile phase, 6.0-7.0; flow rate, 0.8-1.2 mL min(-1) and the ratio of mobile phase composed of a 0.025 M sodium acetate/acetic acid buffer, 80-90%. Resolution was chosen as a response. The optimized method was validated for linearity, the limits of detection and quantification, accuracy, precision and stability. All the validation parameters were within the acceptance range. The applicability of the developed method to the determination of these food additives in commercial lemonade and lemon sauce samples was successfully demonstrated. (© The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.) |
| Databáze: | MEDLINE |
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