Multilocus microsatellite analysis of European and African Candida glabrata isolates.

Autor: Chillemi V; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, 98166, Messina, Italy., Lo Passo C; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, 98166, Messina, Italy., van Diepeningen AD; CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands., Rharmitt S; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, 98166, Messina, Italy., Delfino D; Department of Human Pathology, University of Messina, Via Consolare Valeria, 98125, Messina, Italy., Cascio A; Department of Human Pathology, University of Messina, Via Consolare Valeria, 98125, Messina, Italy., Nnadi NE; Department of Microbiology, Plateau State University, P.M.B 2012, Bokkos, Plateau State, Nigeria., Cilo BD; Medical Microbiology Department, Faculty of Medicine, Uludag University, 16059, Bursa, Turkey., Sampaio P; Biology Department, Minho University, Campus de Gualtar, 4710-057, Braga, Portugal., Tietz HJ; Institut für Pilzkrankheiten, Luisenstrasse 50, 10117, Berlin, Germany., Pemán J; Servicio de Microbiología, Hospital Universitario y Politécnico La Fe, Valencia, España., Criseo G; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, 98166, Messina, Italy., Romeo O; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres, 31, 98166, Messina, Italy. oromeo@unime.it.; IRCCS Centro Neurolesi 'Bonino-Pulejo', SS113, 98124, Messina, Italy. oromeo@unime.it., Scordino F; IRCCS Centro Neurolesi 'Bonino-Pulejo', SS113, 98124, Messina, Italy.
Jazyk: angličtina
Zdroj: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2016 Jun; Vol. 35 (6), pp. 885-92. Date of Electronic Publication: 2016 Mar 05.
DOI: 10.1007/s10096-016-2610-3
Abstrakt: This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.
Databáze: MEDLINE
Externí odkaz: