Downregulation of miR-129-2 by promoter hypermethylation regulates breast cancer cell proliferation and apoptosis.

Autor:	Tang X; Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China., Tang J; Department of Gastroenterology, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, P.R. China., Liu X; Department of Oncology, Guangzhou First Municipal People's Hospital Affiliated to Guangzhou Medical College, Guangzhou, Guangdong 510180, P.R. China., Zeng L; Department of Radiation Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, P.R. China., Cheng C; Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China., Luo Y; Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China., Li L; Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China., Qin SL; Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China., Sang Y; Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China., Deng LM; Department of Medicine, Gaoming Heshui Hospital, Foshan, Guangdong 528500, P.R. China., Lv XB; Nanchang Key Laboratory of Cancer Pathogenesis and Translational Research, Center Laboratory, The Third Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330008, P.R. China.
Jazyk:	angličtina
Zdroj:	Oncology reports [Oncol Rep] 2016 May; Vol. 35 (5), pp. 2963-9. Date of Electronic Publication: 2016 Mar 02.
DOI:	10.3892/or.2016.4647
Abstrakt:	Aberrant expression of the miR-129 family has been found in several types of cancer, yet its expression and potential biologic role in breast cancer remain largely unknown. In the present study, we found that miR-129-2 was consistently downregulated in the breast cancer specimens and cell lines. Overexpression of miR-129-2-3p markedly suppressed breast cancer cell proliferation and induced its apoptosis. In addition, a luciferase reporter assay revealed that miR-129-2-3p suppressed BCL2L2 expression. Furthermore, BCL2L2 was able to reverse miR-129-2-3p-mediated cell apoptosis, indicating that BCL2L2 plays a crucial role in mediating the tumor-suppressive role of miR-129-2-3p. Moreover, bisulfite DNA sequencing PCR (BSP) analysis identified that promoter hypermethylation was responsible for the downregulation of miR-129-2 in breast cancer. Collectively, our findings indicate that miR-129-2 is downregulated in breast cancer cells by promoter hypermethylation. Moreover, downregulation of miR-129-2 results in BCL2L2 overexpression and disease progression in breast cancer patients.
Databáze:	MEDLINE
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