Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets.
Autor: | Bonavita O; Humanitas Clinical and Research Center, Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine, Università degli Studi di Milano, Milano, Italy., Massara M; Humanitas Clinical and Research Center, Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine, Università degli Studi di Milano, Milano, Italy., Anselmo A; Humanitas Clinical and Research Center, Rozzano, Italy., Somma P; Humanitas Clinical and Research Center, Rozzano, Italy., Brühl H; Universitätsklinikum Regensburg, Regensburg, Germany., Mack M; Universitätsklinikum Regensburg, Regensburg, Germany., Locati M; Humanitas Clinical and Research Center, Rozzano, Italy; Department of Medical Biotechnologies and Translational Medicine, Università degli Studi di Milano, Milano, Italy., Bonecchi R; Humanitas Clinical and Research Center, Rozzano, Italy; Department of Biomedical Sciences, Humanitas University, Rozzano, Italy. Electronic address: raffaella.bonecchi@humanitasresearch.it. |
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Jazyk: | angličtina |
Zdroj: | Methods in enzymology [Methods Enzymol] 2016; Vol. 570, pp. 441-56. Date of Electronic Publication: 2015 Nov 24. |
DOI: | 10.1016/bs.mie.2015.09.011 |
Abstrakt: | Chemokine receptors are differentially expressed on leukocyte subpopulations dictating their ability to migrate both in physiological and pathological conditions. Their expression is modulated during leukocyte differentiation and maturation and they can be used as markers to identify and characterize the frequency and the activation state of leukocytes present in a tissue. Here, we will describe flow cytometry approaches to detect chemokine receptors identifying subpopulations of circulating monocytes and neutrophils. (© 2016 Elsevier Inc. All rights reserved.) |
Databáze: | MEDLINE |
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