Large-scale imaging of cortical dynamics during sensory perception and behavior.
| Autor: | Wekselblatt JB; Institute of Neuroscience and Department of Biology, University of Oregon, Eugene, Oregon., Flister ED; Institute of Neuroscience and Department of Biology, University of Oregon, Eugene, Oregon., Piscopo DM; Institute of Neuroscience and Department of Biology, University of Oregon, Eugene, Oregon., Niell CM; Institute of Neuroscience and Department of Biology, University of Oregon, Eugene, Oregon cniell@uoregon.edu. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Journal of neurophysiology [J Neurophysiol] 2016 Jun 01; Vol. 115 (6), pp. 2852-66. Date of Electronic Publication: 2016 Feb 24. |
| DOI: | 10.1152/jn.01056.2015 |
| Abstrakt: | Sensory-driven behaviors engage a cascade of cortical regions to process sensory input and generate motor output. To investigate the temporal dynamics of neural activity at this global scale, we have improved and integrated tools to perform functional imaging across large areas of cortex using a transgenic mouse expressing the genetically encoded calcium sensor GCaMP6s, together with a head-fixed visual discrimination behavior. This technique allows imaging of activity across the dorsal surface of cortex, with spatial resolution adequate to detect differential activity in local regions at least as small as 100 μm. Imaging during an orientation discrimination task reveals a progression of activity in different cortical regions associated with different phases of the task. After cortex-wide patterns of activity are determined, we demonstrate the ability to select a region that displayed conspicuous responses for two-photon microscopy and find that activity in populations of individual neurons in that region correlates with locomotion in trained mice. We expect that this paradigm will be a useful probe of information flow and network processing in brain-wide circuits involved in many sensory and cognitive processes. (Copyright © 2016 the American Physiological Society.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|