A Novel Conductometric Urea Biosensor with Improved Analytical Characteristic Based on Recombinant Urease Adsorbed on Nanoparticle of Silicalite.

Autor: Velychko TP; Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. taras.velychko@gmail.com.; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01003, Kyiv, Ukraine. taras.velychko@gmail.com., Soldatkin ОО; Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. alex_sold@yahoo.com., Melnyk VG; Department of Electrical and Magnetic Measurements, Institute of Electrodynamics of National Academy of Sciences of Ukraine, 56, Peremohy Ave., Kyiv-57, 03680, Ukraine. melnik@gmail.com., Marchenko SV; Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. svmarchenkosv@ukr.net., Kirdeciler SK; Micro and Nanotechnology Department, Middle East Technical University, Ankara, 06531, Turkey. kaankirde@gmail.com.; Central Laboratory, Middle East Technical University, Ankara, 06531, Turkey. kaankirde@gmail.com., Akata B; Micro and Nanotechnology Department, Middle East Technical University, Ankara, 06531, Turkey. akata@metu.edu.tr.; Central Laboratory, Middle East Technical University, Ankara, 06531, Turkey. akata@metu.edu.tr., Soldatkin AP; Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. a_soldatkin@yahoo.com.; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01003, Kyiv, Ukraine. a_soldatkin@yahoo.com., El'skaya AV; Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. elskaya@imbg.org.ua., Dzyadevych SV; Institute of Molecular Biology and Genetics of NAS of Ukraine, Zabolotnogo Street 150, 03143, Kyiv, Ukraine. dzyad@yahoo.com.; Taras Shevchenko National University of Kyiv, Volodymyrska Street 64, 01003, Kyiv, Ukraine. dzyad@yahoo.com.
Jazyk: angličtina
Zdroj: Nanoscale research letters [Nanoscale Res Lett] 2016 Dec; Vol. 11 (1), pp. 106. Date of Electronic Publication: 2016 Feb 25.
DOI: 10.1186/s11671-016-1310-3
Abstrakt: Development of a conductometric biosensor for the urea detection has been reported. It was created using a non-typical method of the recombinant urease immobilization via adsorption on nanoporous particles of silicalite. It should be noted that this biosensor has a number of advantages, such as simple and fast performance, the absence of toxic compounds during biosensor preparation, and high reproducibility (RSD = 5.1 %). The linear range of urea determination by using the biosensor was 0.05-15 mM, and a lower limit of urea detection was 20 μM. The bioselective element was found to be stable for 19 days. The characteristics of recombinant urease-based biomembranes, such as dependence of responses on the protein and ion concentrations, were investigated. It is shown that the developed biosensor can be successfully used for the urea analysis during renal dialysis.
Databáze: MEDLINE
Externí odkaz: