Lipid Profiling of In Vitro Cell Models of Adipogenic Differentiation: Relationships With Mouse Adipose Tissues.

Autor: Liaw L; Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.; The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469., Prudovsky I; Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.; The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469., Koza RA; Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.; The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469., Anunciado-Koza RV; Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074., Siviski ME; Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.; The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469., Lindner V; Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.; The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469., Friesel RE; Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.; The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469., Rosen CJ; Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.; The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469., Baker PR; Sciex Corporation, Framingham, Massachusetts 01701., Simons B; Sciex Corporation, Framingham, Massachusetts 01701., Vary CP; Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, Maine 04074.; The Graduate School of Biomedical Science and Engineering, University of Maine, Orono, Maine 04469.
Jazyk: angličtina
Zdroj: Journal of cellular biochemistry [J Cell Biochem] 2016 Sep; Vol. 117 (9), pp. 2182-93. Date of Electronic Publication: 2016 Mar 16.
DOI: 10.1002/jcb.25522
Abstrakt: Our objective was to characterize lipid profiles in cell models of adipocyte differentiation in comparison to mouse adipose tissues in vivo. A novel lipid extraction strategy was combined with global lipid profiling using direct infusion and sequential precursor ion fragmentation, termed MS/MS(ALL) . Perirenal and inguinal white adipose tissue and interscapular brown adipose tissues from adult C57BL/6J mice were analyzed. 3T3-L1 preadipocytes, ear mesenchymal progenitor cells, and brown adipose-derived BAT-C1 cells were also characterized. Over 3000 unique lipid species were quantified. Principal component analysis showed that perirenal versus inguinal white adipose tissues varied in lipid composition of triacyl- and diacylglycerols, sphingomyelins, glycerophospholipids and, notably, cardiolipin CL 72:3. In contrast, hexosylceramides and sphingomyelins distinguished brown from white adipose. Adipocyte differentiation models showed broad differences in lipid composition among themselves, upon adipogenic differentiation, and with adipose tissues. Palmitoyl triacylglycerides predominate in 3T3-L1 differentiation models, whereas cardiolipin CL 72:1 and SM 45:4 were abundant in brown adipose-derived cell differentiation models, respectively. MS/MS(ALL) data suggest new lipid biomarkers for tissue-specific lipid contributions to adipogenesis, thus providing a foundation for using in vitro models of adipogenesis to reflect potential changes in adipose tissues in vivo. J. Cell. Biochem. 117: 2182-2193, 2016. © 2016 Wiley Periodicals, Inc.
(© 2016 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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