Antimicrobial blue light inactivation of Candida albicans: In vitro and in vivo studies.

Autor: Zhang Y; a Department of Plastic Surgery and Cosmetic , The Second People's Hospital of Guangdong Province , Guangzhou , China.; b Wellman Center for Photomedicine, Massachusetts General Hospital , Boston , MA , USA.; c Department of Dermatology , Harvard Medical School , Boston , MA , USA., Zhu Y; b Wellman Center for Photomedicine, Massachusetts General Hospital , Boston , MA , USA.; d School of Medicine, Tongji University , Shanghai , China., Chen J; b Wellman Center for Photomedicine, Massachusetts General Hospital , Boston , MA , USA.; c Department of Dermatology , Harvard Medical School , Boston , MA , USA.; e Shanghai Dermatology Hospital , Shanghai , China., Wang Y; b Wellman Center for Photomedicine, Massachusetts General Hospital , Boston , MA , USA.; c Department of Dermatology , Harvard Medical School , Boston , MA , USA.; f Department of Laser Medicine , Chinese PLA General Hospital , Beijing , China., Sherwood ME; b Wellman Center for Photomedicine, Massachusetts General Hospital , Boston , MA , USA., Murray CK; g Infectious Disease Service, Brooke Army Medical Center, Fort Sam Houston , TX , USA., Vrahas MS; h Department of orthopedic Surgery , Massachusetts General Hospital, Harvard Medical School , Boston , MA , USA., Hooper DC; i Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School , Boston , MA , USA., Hamblin MR; b Wellman Center for Photomedicine, Massachusetts General Hospital , Boston , MA , USA.; c Department of Dermatology , Harvard Medical School , Boston , MA , USA.; j Harvard-MIT Division of Health Sciences and Technology , Cambridge , MA , USA., Dai T; b Wellman Center for Photomedicine, Massachusetts General Hospital , Boston , MA , USA.; c Department of Dermatology , Harvard Medical School , Boston , MA , USA.
Jazyk: angličtina
Zdroj: Virulence [Virulence] 2016 Jul 03; Vol. 7 (5), pp. 536-45. Date of Electronic Publication: 2016 Feb 24.
DOI: 10.1080/21505594.2016.1155015
Abstrakt: Fungal infections are a common cause of morbidity, mortality and cost in critical care populations. The increasing emergence of antimicrobial resistance necessitates the development of new therapeutic approaches for fungal infections. In the present study, we investigated the effectiveness of an innovative approach, antimicrobial blue light (aBL), for inactivation of Candida albicans in vitro and in infected mouse burns. A bioluminescent strain of C. albicans was used. The susceptibilities to aBL (415 nm) were compared between C. albicans and human keratinocytes. The potential development of aBL resistance by C. albicans was investigated via 10 serial passages of C. albicans on aBL exposure. For the animal study, a mouse model of thermal burn infected with the bioluminescent C. albicans strain was used. aBL was delivered to mouse burns approximately 12 h after fungal inoculation. Bioluminescence imaging was performed to monitor in real time the extent of infection in mice. The results obtained from the studies demonstrated that C. albicans was approximately 42-fold more susceptible to aBL than human keratinocytes. Serial passaging of C. albicans on aBL exposure implied a tendency of reduced aBL susceptibility of C. albicans with increasing numbers of passages; however, no statistically significant difference was observed in the post-aBL survival rate of C. albicans between the first and the last passage (P>0.05). A single exposure of 432 J/cm(2) aBL reduced the fungal burden in infected mouse burns by 1.75-log10 (P=0.015). Taken together, our findings suggest aBL is a potential therapeutic for C. albicans infections.
Databáze: MEDLINE
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