Antimicrobial Blue Light Inactivation of Gram-Negative Pathogens in Biofilms: In Vitro and In Vivo Studies.

Autor:	Wang Y; Department of Laser Medicine, Chinese PLA General Hospital, Beijing College of Medicine, Nankai University, Tianjin Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston., Wu X; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston., Chen J; Shanghai Dermatology Hospital, China Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston., Amin R; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston., Lu M; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston., Bhayana B; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston., Zhao J; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston., Murray CK; Infectious Disease Service, Brooke Army Medical Center, Fort Sam Houston, Texas., Hamblin MR; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston Harvard-MIT Division of Health Sciences and Technology, Cambridge, Massachusetts., Hooper DC; Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Boston., Dai T; Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2016 May 01; Vol. 213 (9), pp. 1380-7. Date of Electronic Publication: 2016 Feb 17.
DOI:	10.1093/infdis/jiw070
Abstrakt:	Background: Biofilms affect >80% bacterial infections in human and are usually difficult to eradicate because of their inherent drug resistance. Methods: We investigated the effectiveness of antimicrobial blue light (aBL) (wavelength, 415 nm) for inactivating Acinetobacter baumannii or Pseudomonas aeruginosa biofilms in 96-well microplates or infected mouse burn wounds. Results: In vitro, in 96-well microplates, exposure of 24-hour-old and 72-hour-old A. baumannii biofilms to 432 J/cm(2) aBL resulted in inactivation of 3.59 log10 and 3.18 log10 colony-forming units (CFU), respectively. For P. aeruginosa biofilms, similar levels of inactivation-3.02 log10 and 3.12 log10 CFU, respectively-were achieved. In mouse burn wounds infected with 5 × 10(6) CFU ofA. baumannii, approximately 360 J/cm(2) and 540 J/cm(2) aBL was required to inactivate 3 log10 CFU in biofilms when delivered 24 and 48 hours, respectively, after bacterial inoculation. High-performance liquid chromatography analysis revealed the presence of endogenous porphyrins in both A. baumannii and P. aeruginosa TUNEL assay detected no apoptotic cells in aBL-irradiated mouse skin at up to 24 hours after aBL exposure (540 J/cm(2)). Conclusions: aBL has antimicrobial activity in biofilms ofA. baumannii and P. aeruginosa and is a potential therapeutic approach for biofilm-related infections. (© The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.)
Databáze:	MEDLINE
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