The dynamics of protein body formation in developing wheat grain.

Autor: Moore KL; School of Materials, University of Manchester, Manchester, UK., Tosi P; School of Agriculture Policy and Development, Reading University, Reading, UK., Palmer R; Rothamsted Research, Harpenden, UK., Hawkesford MJ; Rothamsted Research, Harpenden, UK., Grovenor CR; Department of Materials, University of Oxford, Oxford, UK., Shewry PR; Rothamsted Research, Harpenden, UK.
Jazyk: angličtina
Zdroj: Plant biotechnology journal [Plant Biotechnol J] 2016 Sep; Vol. 14 (9), pp. 1876-82. Date of Electronic Publication: 2016 Mar 15.
DOI: 10.1111/pbi.12549
Abstrakt: Wheat is a major source of protein in the diets of humans and livestock but we know little about the mechanisms that determine the patterns of protein synthesis in the developing endosperm. We have used a combination of enrichment with (15) N glutamine and NanoSIMS imaging to establish that the substrate required for protein synthesis is transported radially from its point of entrance in the endosperm cavity across the starchy endosperm tissues, before becoming concentrated in the cells immediately below the aleurone layer. This transport occurs continuously during grain development but may be slower in the later stages. Although older starchy endosperm cells tend to contain larger protein deposits formed by the fusion of small protein bodies, small highly enriched protein bodies may also be present in the same cells. This shows a continuous process of protein body initiation, in both older and younger starchy endosperm cells and in all regions of the tissue. Immunolabeling with specific antibodies shows that the patterns of enrichment are not related to the contents of gluten proteins in the protein bodies. In addition to providing new information on the dynamics of protein deposition, the study demonstrates the wider utility of NanoSIMS and isotope labelling for studying complex developmental processes in plant tissues.
(© 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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