Label-free electrochemical immunosensor based on Nile blue A-reduced graphene oxide nanocomposites for carcinoembryonic antigen detection.

Autor: Gao YS; College of Science, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China; Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China., Zhu XF; Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China., Xu JK; Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China. Electronic address: xujingkun@tsinghua.org.cn., Lu LM; College of Science, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China. Electronic address: lulimin816@hotmail.com., Wang WM; College of Science, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China., Yang TT; Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China., Xing HK; Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, People's Republic of China., Yu YF; College of Science, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2016 May 01; Vol. 500, pp. 80-7. Date of Electronic Publication: 2016 Feb 18.
DOI: 10.1016/j.ab.2016.02.010
Abstrakt: In this article, a novel, label-free, and inherent electroactive redox immunosensor for carcinoembryonic antigen (CEA) based on gold nanoparticles (AuNPs) and Nile blue A (NB) hybridized electrochemically reduced graphene oxide (NB-ERGO) is proposed. The composite of NB-graphene oxide (NB-GO) was prepared by π-π stacking interaction. Then, chronoamperometry was adopted to simultaneously reduce HAuCl4 and nanocomposites of NB-GO for synthesizing AuNPs/NB-ERGO. The immunosensor was fabricated by capturing CEA antibody (anti-CEA) at this nanocomposite modified electrode. The immunosensor determination was based on the fact that, due to the formation of antigen-antibody immunocomplex, the decreased response currents of NB were directly proportional to the concentrations of CEA. Under optimal conditions, the linear range of the proposed immunosensor was estimated to be from 0.001 to 40 ng ml(-1) and the detection limit was estimated to be 0.00045 ng ml(-1). The proposed immunosensor was used to determine CEA in clinical serum samples with satisfactory results. The proposed method may provide promising potential application in clinical immunoassays with the properties of facile procedure, stability, high sensitivity, and selectivity.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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