Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil.
| Autor: | Cezar RD; Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE), Rua Dom Manuel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, CEP 52171-900, Brazil. natinhacezar@hotmail.com., Lucena-Silva N; Department of Immunology (Departamento de Imunologia), Research Center Aggeu Magalhães (Centro de Pesquisas Aggeu Magalhães-CPqAM) Oswaldo Cruz Foundation (Fundação Oswaldo Cruz - Fiocruz), Av. Professor Moraes Rego, s/n, Cidade Universitária, Recife, Pernambuco, CEP 50.740-465, Brazil. norma.lucena@hotmail.com., Filho AF; Academic Unit of Garanhuns (Unidade Acadêmica de Garanhuns), Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE). Avenida Bom Pastor, s/n, Boa Vista, Garanhuns, Pernambuco, CEP 55292-270, Brazil. afbbf@hotmail.com., Borges Jde M; Academic Unit of Garanhuns (Unidade Acadêmica de Garanhuns), Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE). Avenida Bom Pastor, s/n, Boa Vista, Garanhuns, Pernambuco, CEP 55292-270, Brazil. jonas_borges1@hotmail.com., de Oliveira PR; Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE), Rua Dom Manuel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, CEP 52171-900, Brazil. pollyanne_raysa_fernandes@hotmail.com., Lúcio ÉC; Academic Unit of Garanhuns (Unidade Acadêmica de Garanhuns), Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE). Avenida Bom Pastor, s/n, Boa Vista, Garanhuns, Pernambuco, CEP 55292-270, Brazil. erica.c.l@hotmail.com., Arruda-Lima M; Department of Immunology (Departamento de Imunologia), Research Center Aggeu Magalhães (Centro de Pesquisas Aggeu Magalhães-CPqAM) Oswaldo Cruz Foundation (Fundação Oswaldo Cruz - Fiocruz), Av. Professor Moraes Rego, s/n, Cidade Universitária, Recife, Pernambuco, CEP 50.740-465, Brazil. maira_lima@hotmail.com., Santana VL; National Agricultural Laboratory of Pernambuco (Laboratório Nacional Agropecuário de Pernambuco- Lanagro/PE), Ministry of Agriculture, Livestock and Food Supply of Brazil (Ministério da Agricultura, Pecuária e Abastecimento - MAPA). Rua Manoel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, CEP 52171-030, Brazil. vaniophillus@yahoo.com., Pinheiro Junior JW; Federal Rural University of Pernambuco (Universidade Federal Rural de Pernambuco - UFRPE), Rua Dom Manuel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, CEP 52171-900, Brazil. wiltonjuniorufrpe@gmail.com. |
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| Jazyk: | angličtina |
| Zdroj: | BMC veterinary research [BMC Vet Res] 2016 Feb 20; Vol. 12, pp. 31. Date of Electronic Publication: 2016 Feb 20. |
| DOI: | 10.1186/s12917-016-0656-1 |
| Abstrakt: | Background: The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated. Results: Of the 802 samples analyzed, one milk (0.25%) and eight blood (2%) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30%) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB. Conclusions: M. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil. |
| Databáze: | MEDLINE |
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