A genome-wide resource for the analysis of protein localisation in Drosophila.

Autor: Sarov M; Max Planck Institute of Cell Biology and Genetics, Dresden, Germany., Barz C; Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany., Jambor H; Max Planck Institute of Cell Biology and Genetics, Dresden, Germany., Hein MY; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany., Schmied C; Max Planck Institute of Cell Biology and Genetics, Dresden, Germany., Suchold D; Max Planck Institute of Cell Biology and Genetics, Dresden, Germany., Stender B; Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany., Janosch S; Max Planck Institute of Cell Biology and Genetics, Dresden, Germany., K J VV; Centre for Cellular and Molecular Platforms, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India., Krishnan RT; Centre for Cellular and Molecular Platforms, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India., Krishnamoorthy A; Centre for Cellular and Molecular Platforms, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India., Ferreira IR; Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany., Ejsmont RK; Max Planck Institute of Cell Biology and Genetics, Dresden, Germany., Finkl K; Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany., Hasse S; Max Planck Institute of Cell Biology and Genetics, Dresden, Germany., Kämpfer P; Heidelberg Institute of Theoretical Studies, Heidelberg, Germany., Plewka N; Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany., Vinis E; Max Planck Institute of Cell Biology and Genetics, Dresden, Germany., Schloissnig S; Heidelberg Institute of Theoretical Studies, Heidelberg, Germany., Knust E; Max Planck Institute of Cell Biology and Genetics, Dresden, Germany., Hartenstein V; Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, United States., Mann M; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany., Ramaswami M; Institute of Neuroscience, Trinity College Dublin, Dublin, Ireland., VijayRaghavan K; Centre for Cellular and Molecular Platforms, National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India., Tomancak P; Max Planck Institute of Cell Biology and Genetics, Dresden, Germany., Schnorrer F; Muscle Dynamics Group, Max Planck Institute of Biochemistry, Martinsried, Germany.
Jazyk: angličtina
Zdroj: ELife [Elife] 2016 Feb 20; Vol. 5, pp. e12068. Date of Electronic Publication: 2016 Feb 20.
DOI: 10.7554/eLife.12068
Abstrakt: The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.
Databáze: MEDLINE