Advances and perspectives on the use of CRISPR/Cas9 systems in plant genomics research.

Autor: Liu D; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA., Hu R; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA., Palla KJ; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA., Tuskan GA; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA., Yang X; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6422, USA. Electronic address: yangx@ornl.gov.
Jazyk: angličtina
Zdroj: Current opinion in plant biology [Curr Opin Plant Biol] 2016 Apr; Vol. 30, pp. 70-7. Date of Electronic Publication: 2016 Feb 18.
DOI: 10.1016/j.pbi.2016.01.007
Abstrakt: Genome editing with site-specific nucleases has become a powerful tool for functional characterization of plant genes and genetic improvement of agricultural crops. Among the various site-specific nuclease-based technologies available for genome editing, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems have shown the greatest potential for rapid and efficient editing of genomes in plant species. This article reviews the current status of application of CRISPR/Cas9 to plant genomics research, with a focus on loss-of-function and gain-of-function analysis of individual genes in the context of perennial plants and the potential application of CRISPR/Cas9 to perturbation of gene expression, and identification and analysis of gene modules as part of an accelerated domestication and synthetic biology effort.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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