High-density grids for efficient data collection from multiple crystals.

Autor: Baxter EL; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Aguila L; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Alonso-Mori R; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Barnes CO; Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Bonagura CA; Art Robbins Instruments, Sunnyvale, CA 94089, USA., Brehmer W; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Brunger AT; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., Calero G; Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA., Caradoc-Davies TT; The ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, Melbourne, Victoria 3800, Australia., Chatterjee R; Physical Bioscences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Degrado WF; Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA., Fraser JS; Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA 94158, USA., Ibrahim M; Institut für Biologie, Humboldt-Universität zu Berlin, 10099 Berlin, Germany., Kern J; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Kobilka BK; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., Kruse AC; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., Larsson KM; Stanford University School of Medicine, Stanford, CA 94305, USA., Lemke HT; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Lyubimov AY; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., Manglik A; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., McPhillips SE; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Norgren E; Art Robbins Instruments, Sunnyvale, CA 94089, USA., Pang SS; The ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, Melbourne, Victoria 3800, Australia., Soltis SM; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Song J; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Thomaston J; Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA., Tsai Y; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA., Weis WI; Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305, USA., Woldeyes RA; Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, CA 94158, USA., Yachandra V; Physical Bioscences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Yano J; Physical Bioscences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA., Zouni A; Institut für Biologie, Humboldt-Universität zu Berlin, 10099 Berlin, Germany., Cohen AE; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.
Jazyk: angličtina
Zdroj: Acta crystallographica. Section D, Structural biology [Acta Crystallogr D Struct Biol] 2016 Jan; Vol. 72 (Pt 1), pp. 2-11. Date of Electronic Publication: 2016 Jan 01.
DOI: 10.1107/S2059798315020847
Abstrakt: Higher throughput methods to mount and collect data from multiple small and radiation-sensitive crystals are important to support challenging structural investigations using microfocus synchrotron beamlines. Furthermore, efficient sample-delivery methods are essential to carry out productive femtosecond crystallography experiments at X-ray free-electron laser (XFEL) sources such as the Linac Coherent Light Source (LCLS). To address these needs, a high-density sample grid useful as a scaffold for both crystal growth and diffraction data collection has been developed and utilized for efficient goniometer-based sample delivery at synchrotron and XFEL sources. A single grid contains 75 mounting ports and fits inside an SSRL cassette or uni-puck storage container. The use of grids with an SSRL cassette expands the cassette capacity up to 7200 samples. Grids may also be covered with a polymer film or sleeve for efficient room-temperature data collection from multiple samples. New automated routines have been incorporated into the Blu-Ice/DCSS experimental control system to support grids, including semi-automated grid alignment, fully automated positioning of grid ports, rastering and automated data collection. Specialized tools have been developed to support crystallization experiments on grids, including a universal adaptor, which allows grids to be filled by commercial liquid-handling robots, as well as incubation chambers, which support vapor-diffusion and lipidic cubic phase crystallization experiments. Experiments in which crystals were loaded into grids or grown on grids using liquid-handling robots and incubation chambers are described. Crystals were screened at LCLS-XPP and SSRL BL12-2 at room temperature and cryogenic temperatures.
Databáze: MEDLINE