Simultaneous quantitative analysis of nine vitamin D compounds in human blood using LC-MS/MS.

Autor:	Abu Kassim NS; School of Pharmacy, The University of Queensland, QLD 4072, Australia., Gomes FP; School of Pharmacy, The University of Queensland, QLD 4072, Australia., Shaw PN; School of Pharmacy, The University of Queensland, QLD 4072, Australia., Hewavitharana AK; School of Pharmacy, The University of Queensland, QLD 4072, Australia.
Jazyk:	angličtina
Zdroj:	Bioanalysis [Bioanalysis] 2016; Vol. 8 (5), pp. 397-411. Date of Electronic Publication: 2016 Feb 19.
DOI:	10.4155/bio.15.260
Abstrakt:	Aim: It has been suggested that each member of the family of vitamin D compounds may have different function(s). Therefore, selective quantification of each compound is important in clinical research. Materials & Methods: Development and validation attempts of a simultaneous determination method of 12 vitamin D compounds in human blood using precolumn derivatization followed by LC-MS/MS is described. Internal standard calibration with 12 stable isotope labeled analogs was used to correct for matrix effects in MS detector. Results & Conclusion: Nine vitamin D compounds were quantifiable in blood samples with detection limits within femtomole levels. Serum (compared with plasma) was found to be a more suitable sample type, and protein precipitation (compared with saponification) a more effective extraction method for vitamin D assay.
Databáze:	MEDLINE
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