Potential of genetically engineered hybrid poplar for pyrolytic production of bio-based phenolic compounds.

Autor: Toraman HE; Ghent University, Laboratory for Chemical Technology, Technologiepark 914, 9052 Ghent, Belgium., Vanholme R; Ghent University, Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Ghent, Belgium., Borén E; Ghent University, Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Ghent, Belgium; Umeå University, Department of Applied Physics and Electronics, 901 87 Umeå, Sweden., Vanwonterghem Y; Ghent University, Laboratory for Chemical Technology, Technologiepark 914, 9052 Ghent, Belgium., Djokic MR; Ghent University, Laboratory for Chemical Technology, Technologiepark 914, 9052 Ghent, Belgium., Yildiz G; Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Ghent, Belgium., Ronsse F; Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Ghent, Belgium., Prins W; Ghent University, Department of Biosystems Engineering, Coupure Links 653, 9000 Ghent, Belgium., Boerjan W; Ghent University, Department of Plant Systems Biology, VIB, Technologiepark 927, 9052 Ghent, Belgium., Van Geem KM; Ghent University, Laboratory for Chemical Technology, Technologiepark 914, 9052 Ghent, Belgium. Electronic address: Kevin.VanGeem@UGent.be., Marin GB; Ghent University, Laboratory for Chemical Technology, Technologiepark 914, 9052 Ghent, Belgium.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2016 May; Vol. 207, pp. 229-36. Date of Electronic Publication: 2016 Feb 09.
DOI: 10.1016/j.biortech.2016.02.022
Abstrakt: Wild-type and two genetically engineered hybrid poplar lines were pyrolyzed in a micro-pyrolysis (Py-GC/MS) and a bench scale setup for fast and intermediate pyrolysis studies. Principal component analysis showed that the pyrolysis vapors obtained by micro-pyrolysis from wood of caffeic acid O-methyltransferase (COMT) and caffeoyl-CoA O-methyltransferase (CCoAOMT) down-regulated poplar trees differed significantly from the pyrolysis vapors obtained from non-transgenic control trees. Both fast micro-pyrolysis and intermediate pyrolysis of transgenic hybrid poplars showed that down-regulation of COMT can enhance the relative yield of guaiacyl lignin-derived products, while the relative yield of syringyl lignin-derived products was up to a factor 3 lower. This study indicates that lignin engineering via genetic modifications of genes involved in the phenylpropanoid and monolignol biosynthetic pathways can help to steer the pyrolytic production of guaiacyl and syringyl lignin-derived phenolic compounds such as guaiacol, 4-methylguaiacol, 4-ethylguaiacol, 4-vinylguaiacol, syringol, 4-vinylsyringol, and syringaldehyde present in the bio-oil.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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