Protection against oxidative stress by vitamin D in cone cells.

Autor: Tohari AM; Department of Life Sciences, Glasgow Caledonian University, Glasgow, UK.; King Fahad Hospital, Jazan, Saudi Arabia., Zhou X; Department of Life Sciences, Glasgow Caledonian University, Glasgow, UK., Shu X; Department of Life Sciences, Glasgow Caledonian University, Glasgow, UK.
Jazyk: angličtina
Zdroj: Cell biochemistry and function [Cell Biochem Funct] 2016 Mar; Vol. 34 (2), pp. 82-94. Date of Electronic Publication: 2016 Feb 17.
DOI: 10.1002/cbf.3167
Abstrakt: Photoreceptor degeneration (PD) refers to a group of heterogeneous outer retinal dystrophies characterized by the death of photoreceptors. Both oxidative stress and inflammation are involved in the pathogenesis of PD. We investigate whether vitamin D has a potential for the treatment of PD by evaluating the anti-oxidative stress and anti-inflammatory properties of the active form of vitamin D3 , 1,α, 25-dihydroxyvitamin D3 , in a mouse cone cell line, 661W. Mouse cone cells were treated with H2 O2 or a mixture of H2 O2 and vitamin D; cell viability was determined. The production of reactive oxygen species (ROS) in treated and untreated cells was measured. The expression of key anti-oxidative stress and inflammatory genes in treated and untreated cells was determined. Treatment with vitamin D significantly increased cell viability and decreased ROS production in 661W cells under oxidative stress induced by H2 O2 . H2 O2 treatment in 661W cells can significantly down-regulate the expression of antioxidant genes and up-regulate the expression of neurotoxic cytokines. Vitamin D treatment significantly reversed these effects and restored the expression of antioxidant genes. Vitamin D treatment also can block H2 O2 induced oxidative damages. The data suggested that vitamin D may offer a therapeutic potential for patients with PD.
(Copyright © 2016 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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