Energy restriction impairs dendritic cell development in C57BL/6J mice.

Autor: Duriancik DM; Department of Food Science & Human Nutrition, Michigan State University, East Lansing, MI 48824-1224, United States., Gardner EM; Department of Food Science & Human Nutrition, Michigan State University, East Lansing, MI 48824-1224, United States. Electronic address: egardner@msu.edu.
Jazyk: angličtina
Zdroj: Mechanisms of ageing and development [Mech Ageing Dev] 2016 Mar; Vol. 154, pp. 9-19. Date of Electronic Publication: 2016 Feb 10.
DOI: 10.1016/j.mad.2016.02.002
Abstrakt: Dendritic cells (DC) are antigen-presenting cells known for stimulating naïve T lymphocytes. The sequential stages of DC development from common myeloid progenitors have been elucidated in murine bone marrow. Energy-restriction (ER) is a pro-longevity dietary intervention with mixed immunological outcomes. The objective of this study was to examine the development of DC in adult C57Bl6J mice fed a 40% ER diet. We observed increased myeloid progenitors, but decreased common DC progenitors, precursor conventional DC and plasmacytoid DC. Furthermore, we observed increased macrophages and cells expressing CD169 in the bone marrow of ER mice. There was no significant difference in DC subsets from unfractionated ER and ad libitum-fed murine bone marrow samples cultured in GM-CSF-supplemented media or Flt3L-supplemented media. Examining rates of proliferation with 6h BrdU incorporation and Ki-67 staining showed these DC progenitor populations have different proliferation rates in ER compared with AL mice. We show here, for the first time, ER results in altered myelopoiesis resulting in reduced DC development but enhanced monocyte/macrophage development in steady-state C57Bl6J mice. In conclusion, these data may partially explain prior observations of impaired early innate immune responses to primary infection such as influenza in ER mice.
(Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.)
Databáze: MEDLINE
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