Use of chromatin remodeling ATPases as RNAi targets for parental control of western corn rootworm (Diabrotica virgifera virgifera) and Neotropical brown stink bug (Euschistus heros).

Autor: Fishilevich E; Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States., Vélez AM; University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States., Khajuria C; University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States., Frey ML; Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States., Hamm RL; Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States., Wang H; University of Nebraska, Department of Entomology, 103 Entomology Hall, Lincoln, NE 68583-0816, United States., Schulenberg GA; Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States., Bowling AJ; Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States., Pence HE; Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States., Gandra P; Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States., Arora K; Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States., Storer NP; Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States., Narva KE; Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN, United States., Siegfried BD; Entomology and Nematology Department, Charles Steinmetz Hall, University of Florida, P.O. Box 110620, Gainesville, FL 32611-0620, United States. Electronic address: bsiegfried1@ufl.edu.
Jazyk: angličtina
Zdroj: Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2016 Apr; Vol. 71, pp. 58-71. Date of Electronic Publication: 2016 Feb 10.
DOI: 10.1016/j.ibmb.2016.02.004
Abstrakt: RNA interference (RNAi) is a gene silencing mechanism that is present in animals and plants and is triggered by double stranded RNA (dsRNA) or small interfering RNA (siRNA), depending on the organism. In the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), RNAi can be achieved by feeding rootworms dsRNA added to artificial diet or plant tissues transformed to express dsRNA. The effect of RNAi depends on the targeted gene function and can range from an absence of phenotypic response to readily apparent responses, including lethality. Furthermore, RNAi can directly affect individuals that consume dsRNA or the effect may be transferred to the next generation. Our previous work described the potential use of genes involved in embryonic development as a parental RNAi technology for the control of WCR. In this study, we describe the use of chromatin-remodeling ATPases as target genes to achieve parental gene silencing in two insect pests, a coleopteran, WCR, and a hemipteran, the Neotropical brown stink bug, Euschistus heros Fabricius (Hemiptera: Pentatomidae). Our results show that dsRNA targeting chromatin-remodeling ATPase transcripts, brahma, mi-2, and iswi strongly reduced the fecundity of the exposed females in both insect species. Additionally, knockdown of chd1 reduced the fecundity of E. heros.
(Copyright © 2016 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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