Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells.

Autor:	Souza CM; Laboratório de Pesquisa em Microbiologia Aplicada, Universidade de Franca, Franca, SP, Brazil., Pereira Junior SA; Laboratório de Pesquisa em Microbiologia Aplicada, Universidade de Franca, Franca, SP, Brazil., Moraes Tda S; Laboratório de Pesquisa em Microbiologia Aplicada, Universidade de Franca, Franca, SP, Brazil., Damasceno JL; Laboratório de Pesquisa em Microbiologia Aplicada, Universidade de Franca, Franca, SP, Brazil., Amorim Mendes S; Laboratório de Pesquisa em Microbiologia Aplicada, Universidade de Franca, Franca, SP, Brazil., Dias HJ; Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil., Stefani R; Instituto de Ciências Exatas e da Terra, Universidade Federal do Mato Grosso, Pontal do Araguaia, MT, Brazil., Tavares DC; Laboratório de Pesquisa em Microbiologia Aplicada, Universidade de Franca, Franca, SP, Brazil., Martins CH; Laboratório de Pesquisa em Microbiologia Aplicada, Universidade de Franca, Franca, SP, Brazil., Crotti AE; Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil., Mendes-Giannini MJ; Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, São Paulo, Brazil., Pires RH; Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara, São Paulo, Brazil rehepi@gmail.com.
Jazyk:	angličtina
Zdroj:	Medical mycology [Med Mycol] 2016 Jul 01; Vol. 54 (5), pp. 515-23. Date of Electronic Publication: 2016 Feb 11.
DOI:	10.1093/mmy/myw003
Abstrakt:	Dental prosthesis supports Candida species growth and may predispose the oral cavity to lesions. C. tropicalis has emerged as a colonizer of prosthesis and has shown resistance to clinically used antifungal agents, which has increased the search for new antifungals. This work describes the effectiveness of fifteen essential oils (EOs) against C. tropicalis The EOs were obtained by hydrodistillation and were chemically characterized by gas chromatography-mass spectrometry. The antifungal activities of the EOs were evaluated by the microdilution method and showed that Pelargonium graveolens (Geraniaceae) (PG-EO) was the most effective oil. Geraniol and linalool were the major constituents of PG-EO. The 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay showed that all the clinical C. tropicalis strains formed viable biofilms. Scanning electron microscopy examination of the biofilms revealed a complex architecture with basal layer of yeast cells and an upper layer of filamentous cells. Treatments with PG-EO, linalool, and geraniol significantly reduced the number of viable biofilm cells and inhibited biofilm formation after exposure for 48 h. PG-EO, geraniol, and linalool were not toxic to normal human lung fibroblasts (GM07492A) at the concentrations they were active against C. tropicalis Together, our results indicated that C. tropicalis is susceptible to treatment with PG-EO, geraniol, and linalool, which could become options to prevent or treat this infection. (© The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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