| Autor: |
Szkaradkiewicz A; Department of Medical Microbiology, University of Medical Sciences in Poznań, Wieniawskiego 3, Str., 61-712, Poznań, Poland., Karpiński TM; Department of Medical Microbiology, University of Medical Sciences in Poznań, Wieniawskiego 3, Str., 61-712, Poznań, Poland., Linke K; Department of Gastroenterology, Human Nutrition and Internal Diseases, University of Medical Sciences in Poznań, Przybyszewskiego 49, Str., 60-355, Poznań, Poland., Majewski P; Department of Clinical Pathomorphology, University of Medical Sciences in Poznań, Przybyszewskiego 49, Str., 60-355, Poznań, Poland., Rożkiewicz D; Department of Paediatric Infectious Diseases, Medical University of Białystok, University Children's Hospital, Waszyngtona 17, Str., 15-274, Białystok, Poland., Goślińska-Kuźniarek O; Department of Medical Microbiology, University of Medical Sciences in Poznań, Wieniawskiego 3, Str., 61-712, Poznań, Poland. |
| Abstrakt: |
In order to better understand pathogenicity of Helicobacter pylori, particularly in the context of its carcinogenic activity, we analysed expression of virulence genes: cagA, virB/D complex (virB4, virB7, virB8, virB9, virB10, virB11, virD4) and vacA in strains of the pathogen originating from persons with gastric diseases. The studies were conducted on 42 strains of H. pylori isolated from patients with histological diagnosis of non-atrophic gastritis-NAG (group 1, including subgroup 1 containing cagA+ isolates and subgroup 2 containing cagA- strains), multifocal atrophic gastritis-MAG (group 2) and gastric adenocarcinoma-GC (group 3). Expression of H. pylori genes was studied using microarray technology. In group 1, in all strains of H. pylori cagA+ (subgroup 1) high expression of the gene as well as of virB/D was disclosed, accompanied by moderate expression of vacA. In strains of subgroup 2 a moderate expression of vacA was detected. All strains in groups 2 and 3 carried cagA gene but they differed in its expression: a high expression was detected in isolates of group 2 and its hyperexpression in strains of group 3 (hypervirulent strains). In both groups high expression of virB/D and vacA was disclosed. Our results indicate that chronic active gastritis may be induced by both cagA+ strains of H. pylori, manifesting high expression of virB/D complex but moderate activity of vacA, and cagA- strains with moderate expression of vacA gene. On the other hand, in progression of gastric pathology and carcinogenesis linked to H. pylori a significant role was played by hypervirulent strains, manifesting a very high expression of cagA and high activity of virB/D and vacA genes. |
