Nanoscale Metal-Organic Frameworks for Ratiometric Oxygen Sensing in Live Cells.

Autor: Xu R; Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States., Wang Y; Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States.; Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology , Shanghai 200237, China., Duan X; Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States., Lu K; Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States., Micheroni D; Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States., Hu A; Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology , Shanghai 200237, China., Lin W; Department of Chemistry, University of Chicago , 929 East 57th Street, Chicago, Illinois 60637, United States.
Jazyk: angličtina
Zdroj: Journal of the American Chemical Society [J Am Chem Soc] 2016 Feb 24; Vol. 138 (7), pp. 2158-61. Date of Electronic Publication: 2016 Feb 16.
DOI: 10.1021/jacs.5b13458
Abstrakt: We report the design of a phosphorescence/fluorescence dual-emissive nanoscale metal-organic framework (NMOF), R-UiO, as an intracellular oxygen (O2) sensor. R-UiO contains a Pt(II)-porphyrin ligand as an O2-sensitive probe and a Rhodamine-B isothiocyanate ligand as an O2-insensitive reference probe. It exhibits good crystallinity, high stability, and excellent ratiometric luminescence response to O2 partial pressure. In vitro experiments confirmed the applicability of R-UiO as an intracellular O2 biosensor. This work is the first report of a NMOF-based intracellular oxygen sensor and should inspire the design of ratiometric NMOF sensors for other important analytes in biological systems.
Databáze: MEDLINE
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