Bone biology-related gingival transcriptome in ageing and periodontitis in non-human primates.

Autor: Pandruvada SN; Division of Orthodontics, College of Dentistry, University of Kentucky, Lexington, KY, USA.; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA., Gonzalez OA; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA., Kirakodu S; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA., Gudhimella S; Division of Orthodontics, College of Dentistry, University of Kentucky, Lexington, KY, USA., Stromberg AJ; Department of Statistics, University of Kentucky, Lexington, KY, USA., Ebersole JL; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA., Orraca L; School of Dental Medicine, University of Puerto Rico, San Juan, PR, USA., Gonzalez-Martinez J; Caribbean Primate Research Center, Sabana Seca, PR, USA., Novak MJ; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA., Huja SS; Division of Orthodontics, College of Dentistry, University of Kentucky, Lexington, KY, USA.; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA.
Jazyk: angličtina
Zdroj: Journal of clinical periodontology [J Clin Periodontol] 2016 May; Vol. 43 (5), pp. 408-17. Date of Electronic Publication: 2016 Apr 06.
DOI: 10.1111/jcpe.12528
Abstrakt: Aim: Cellular and molecular immunoinflammatory changes in gingival tissues drive alveolar bone loss in periodontitis. Since ageing is a risk factor for periodontitis, we sought to identify age-related gingival transcriptome changes associated with bone metabolism in both healthy and in naturally occurring periodontitis.
Materials and Methods: Adult (12-16 years) and aged (18-23 years) non-human primates (M. mulatta) (n = 24) were grouped into healthy and periodontitis. Gingival tissue samples were obtained and subjected to microarray analysis using the Gene Chip Macaque Genome Array. Gene expression profiles involved in osteoclast/osteoblast proliferation, adhesion and function were evaluated and compared across and between the age groups. QPCR was also performed on selected genes to validate microarray data.
Results: Healthy aged tissues showed a gene profile expression that suggest enhancement of osteoclastic adhesion, proliferation/survival and function (SPP1, TLR4, MMP8 and TFEC) and impaired osteoblastic activity (SMEK3P and SMAD5). The gingival transcriptome in both adult and aged animals with naturally occurring periodontitis (FOS, IL6, TLR4, MMP9, MMP10 and SPP1 genes) was consistent with a local inflammatory response driving towards bone/connective tissue destruction.
Conclusion: A pro-osteoclastogenic gingival transcriptome is associated with periodontitis irrespective of age; however; a greater bone-destructive molecular environment is associated with ageing in healthy tissues.
(© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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